中华口腔医学研究杂志(电子版)
中華口腔醫學研究雜誌(電子版)
중화구강의학연구잡지(전자판)
CHINESE JOURNAL OF STOMATOLOGICAL RESEARCH(ELECTRONIC VERSION)
2015年
2期
118-122
,共5页
陈晓丹%向映辉%覃峰%杨志%谭淑仪%宋子珺%付强
陳曉丹%嚮映輝%覃峰%楊誌%譚淑儀%宋子珺%付彊
진효단%향영휘%담봉%양지%담숙의%송자군%부강
血小板源性生长因子%流体剪切力%成骨细胞%c-fos%增殖
血小闆源性生長因子%流體剪切力%成骨細胞%c-fos%增殖
혈소판원성생장인자%류체전절력%성골세포%c-fos%증식
Platelet-derived growth factor%Fluid shear stress%Osteoblasts%c-fos%Proliferation
目的:研究血小板源性生长因子(PDGF)与流体剪切力(FSS)对成骨细胞增殖及c-fos表达的影响。方法将0、10、30和50 ng/ml的PDGF作用于成骨细胞,CCK-8、实时荧光定量聚合酶链反应(PCR)分别检测细胞增殖和c-fos mRNA表达;将PDGF作用于成骨细胞,同时以12 dyne/cm2的FSS加载1 h,分别采用CCK-8、实时荧光定量PCR和Western blot检测成骨细胞增殖、c-fos mRNA和蛋白的表达。结果 PDGF或FSS单独作用均能促进成骨细胞增殖(FFSS=6.500,PFSS<0.05;FPDGF=6.077,PPDGF<0.05),并使c-fos mRNA表达水平显著升高(FFSS=6.425,PFSS<0.05;FPDGF=7.549,PPDGF<0.05);但PDGF与FSS间不存在协同作用(F 增殖=1.826,P 增殖>0.05;Fc-fos mRNA=2.101,Pc-fos mRNA>0.05;Fc-fos 蛋白=1.561,Pc-fos 蛋白>0.05)。结论 PDGF单独作用或与FSS联合应用均可促进成骨细胞增殖和c-fos基因的表达,但PDGF与FSS间不存在协同作用。
目的:研究血小闆源性生長因子(PDGF)與流體剪切力(FSS)對成骨細胞增殖及c-fos錶達的影響。方法將0、10、30和50 ng/ml的PDGF作用于成骨細胞,CCK-8、實時熒光定量聚閤酶鏈反應(PCR)分彆檢測細胞增殖和c-fos mRNA錶達;將PDGF作用于成骨細胞,同時以12 dyne/cm2的FSS加載1 h,分彆採用CCK-8、實時熒光定量PCR和Western blot檢測成骨細胞增殖、c-fos mRNA和蛋白的錶達。結果 PDGF或FSS單獨作用均能促進成骨細胞增殖(FFSS=6.500,PFSS<0.05;FPDGF=6.077,PPDGF<0.05),併使c-fos mRNA錶達水平顯著升高(FFSS=6.425,PFSS<0.05;FPDGF=7.549,PPDGF<0.05);但PDGF與FSS間不存在協同作用(F 增殖=1.826,P 增殖>0.05;Fc-fos mRNA=2.101,Pc-fos mRNA>0.05;Fc-fos 蛋白=1.561,Pc-fos 蛋白>0.05)。結論 PDGF單獨作用或與FSS聯閤應用均可促進成骨細胞增殖和c-fos基因的錶達,但PDGF與FSS間不存在協同作用。
목적:연구혈소판원성생장인자(PDGF)여류체전절력(FSS)대성골세포증식급c-fos표체적영향。방법장0、10、30화50 ng/ml적PDGF작용우성골세포,CCK-8、실시형광정량취합매련반응(PCR)분별검측세포증식화c-fos mRNA표체;장PDGF작용우성골세포,동시이12 dyne/cm2적FSS가재1 h,분별채용CCK-8、실시형광정량PCR화Western blot검측성골세포증식、c-fos mRNA화단백적표체。결과 PDGF혹FSS단독작용균능촉진성골세포증식(FFSS=6.500,PFSS<0.05;FPDGF=6.077,PPDGF<0.05),병사c-fos mRNA표체수평현저승고(FFSS=6.425,PFSS<0.05;FPDGF=7.549,PPDGF<0.05);단PDGF여FSS간불존재협동작용(F 증식=1.826,P 증식>0.05;Fc-fos mRNA=2.101,Pc-fos mRNA>0.05;Fc-fos 단백=1.561,Pc-fos 단백>0.05)。결론 PDGF단독작용혹여FSS연합응용균가촉진성골세포증식화c-fos기인적표체,단PDGF여FSS간불존재협동작용。
Objectives To study the effect of platelet-derived growth factor (PDGF) and fluid shear stress (FSS) on cell proliferation and c-fos expression in osteoblasts. Methods Different concerntrations of PDGF (0, 10, 30, 50 ng/ml) were used to stimulate primary cultured osteoblasts, respectively. CCK-8 and real-time quantitative PCR were performed to detect cell proliferation and c-fos mRNA expression . Furthermore, osteoblasts were stimulated by PDGF and loaded by FSS of 12 dyne/cm2 for 1 h, together. CCK-8, real-time quantitative PCR and western blot were performed to detect cell proliferation , c-fos mRNA and protein expression, respectively. Results PDGF or FSS can respectively increase cell proliferation (FFSS=6.500,PFSS<0.05;FPDGF=6.077,PPDGF<0.05) and the expression levels of c-fos mRNA in osteoblasts(FFSS=6.425,PFSS<0.05;FPDGF=7.549,PPDGF<0.05). There was no synergistic effect between PDGF and FSS (Fproliferation=1.826,Pproliferation>0.05;Fc-fos mRNA=2.101,Pc-fos mRNA>0.05;Fc-fos protein=1.561,Pc-fos protein>0.05). Conclusion PDGF itself or combined with FSS can increase cell proliferation and c-fos expression levels in osteoblasts, but there is no synergistic effect between PDGF and FSS on cell proliferation and c-fos expression in osteoblasts.