CAJ | 학술논문

目的：探讨叶酸（FA）介导的超顺磁性氧化铁纳米颗粒（PEG-PCL-SPIO）对于人舌癌细胞Tca-8113的靶向性及磁共振成像（MRI）的可行性。方法制备PEG-PCL-SPIO探针及FA靶向修饰的探针PEG-PCL-FA-SPIO，通过MTT试验验证其细胞毒性，并分别用PEG-PCL-SPIO（非靶向组）、PEG-PCL-FA-SPIO（靶向组）与人舌癌细胞Tca-8113（1×106）共同培育0.5、1、2 h后做细胞悬液的MRI检查，测量横向弛豫时间（T2值）变化。采用普鲁士蓝染色观察SPIO进入细胞内的情况。结果 MTT比色试验培养液中不同浓度的PEG-PCL-FA-SPIO的细胞活性，各组间的差异无统计学意义（F＝0.698，P＝0.676）。两种探针分别与Tca-8113细胞培育后0.5、1、2 h后MRI检查，两组细胞悬液的T2加权成像信号均下降，以2 h后更明显，靶向组在各时间段T2值下降的百分比均高于非靶向组（F＝518.064，P＝0.000）。普鲁士蓝染色显示，两种标记的细胞内均可见蓝色颗粒，靶向组中的蓝色颗粒比非靶向组的蓝色颗粒更多。结论 PEG-PCL-FA-SPIO对Tca-8113细胞有良好的靶向性，对舌癌的早期诊断具有重大应用潜力。
목적：탐토협산（FA）개도적초순자성양화철납미과립（PEG-PCL-SPIO）대우인설암세포Tca-8113적파향성급자공진성상（MRI）적가행성。방법제비PEG-PCL-SPIO탐침급FA파향수식적탐침PEG-PCL-FA-SPIO，통과MTT시험험증기세포독성，병분별용PEG-PCL-SPIO（비파향조）、PEG-PCL-FA-SPIO（파향조）여인설암세포Tca-8113（1×106）공동배육0.5、1、2 h후주세포현액적MRI검사，측량횡향이예시간（T2치）변화。채용보로사람염색관찰SPIO진입세포내적정황。결과 MTT비색시험배양액중불동농도적PEG-PCL-FA-SPIO적세포활성，각조간적차이무통계학의의（F＝0.698，P＝0.676）。량충탐침분별여Tca-8113세포배육후0.5、1、2 h후MRI검사，량조세포현액적T2가권성상신호균하강，이2 h후경명현，파향조재각시간단T2치하강적백분비균고우비파향조（F＝518.064，P＝0.000）。보로사람염색현시，량충표기적세포내균가견람색과립，파향조중적람색과립비비파향조적람색과립경다。결론 PEG-PCL-FA-SPIO대Tca-8113세포유량호적파향성，대설암적조기진단구유중대응용잠력。
Objective To explore the feasibility of the human tongue cancer cell (Tca-8113) targeting and magnetic resonance imaging (MRI) abilities of the folate mediated superparamagnetic iron oxide nanoparticles (PEG-PCL-FA-SPIO). Methods Both PEG-PCL-SPIO probes and PEG-PCL-FA-SPIO probes with folate targeted modification were prepared . The cytotoxicity of PEG-PCL-FA-SPIO was verified through MTT assay. Tca-8113 cell (1 × 106) were cultured with PEG-PCL-SPIO (non-targeting) and PEG-PCL-FA-SPIO (folate targeting), respectively. Each group underwent MRI scan after cultured for 0.5, 1, 2 h, and the relative T2 values were recorded. Endocytosis of SPIO was observed through prussian blue staining. Results The cell viability of PEG-PCL-FA-SPIO in different concentrations showed no statistical difference according to the MTT assay (F=0.698, P=0.676). Both the two probes were cultured with Tca-8113 cells for 0.5, 1, 2 h, respectively and the MRI demonstrated decreased T2WI signal intensity of both groups, especially at 2 h. The folate targeting group showed a more significant decrease of relative T2 value at each time point (F=518.064, P=0.000). Intracellular blue particles were observed in both groups after prussian blue staining , and the particles in folate targeting group were more than that of the non-targeting group . Conclusion PEG-PCL-FA-SPIO has favourable Tca-8113 cell targeting ability, and shows great potential in the early diagnosis of tongue cancer.