CAJ | 학술논문

对广粉1号粉蕉腋芽的消毒方法、外植体诱导、幼芽增殖、生根培养进行研究。结果表明，采用75%酒精60 s+0.1%升汞(第1次10 min+第2次10 min)的消毒方法，比较适于广粉1号粉蕉外植体消毒，易获得理想的无菌体系；在不定芽的诱导初期阶段，6-BA 浓度采用5.0 mg/L，最适合广粉1号粉蕉外植体的诱导；在幼芽的中高代增殖培养阶段，适宜采用 MS+6-BA 4.0 mg/L+AD 3.0 mg/L+NAA 0.1 mg/L 配方；在幼芽的高代增殖培养阶段，适宜采用 MS+6-BA 3.0 mg/L+AD 3.0 mg/L+NAA 0.1 mg/L 配方；在幼芽的生根阶段，适宜采用 MS+IBA 2.5 mg/L+NAA 0.2 mg/L 配方，幼苗生根快、根系壮实、发达，成活率高。
대엄분1호분초액아적소독방법、외식체유도、유아증식、생근배양진행연구。결과표명，채용75%주정60 s+0.1%승홍(제1차10 min+제2차10 min)적소독방법，비교괄우엄분1호분초외식체소독，역획득이상적무균체계；재불정아적유도초기계단，6-BA 농도채용5.0 mg/L，최괄합엄분1호분초외식체적유도；재유아적중고대증식배양계단，괄의채용 MS+6-BA 4.0 mg/L+AD 3.0 mg/L+NAA 0.1 mg/L 배방；재유아적고대증식배양계단，괄의채용 MS+6-BA 3.0 mg/L+AD 3.0 mg/L+NAA 0.1 mg/L 배방；재유아적생근계단，괄의채용 MS+IBA 2.5 mg/L+NAA 0.2 mg/L 배방，유묘생근쾌、근계장실、발체，성활솔고。
The sterilize method, induction of explant and multiplication of bud and rooting culture for dwarf banana bud were studied. The results indicated the sterile treatment of 75% alcohol for 60 seconds+0.1% Hgcl2 (first 10 min + second time 10min) is more appropriate in sterilize of explants for dwarf banana, it was easy to obtain ideal aseptic system with the method. 6-BA 5.0 mg/L was suitable for the inductions in the early stages of bud induction. MS+6- BA 4.0 mg/L+AD 3.0 mg/L+NAA 0.1 mg/L was suitable in the stage of middle and high proliferation culture for bud. MS+6-BA 3.0 mg/L+AD 3.0 mg/L+NAA 0.1 mg/L was suitable in the stage of high proliferation culture for bud. MS+IBA 2.5 mg/L+NAA 0.2 mg/L was suitable in shoots rooting stage of bud. Seedling rooted quickly, and roots were stout with high survival rate.