CAJ | 학술논문

目的：观察高糖状态对人牙龈上皮细胞生物学特性的影响。方法原代培养人牙龈上皮细胞（hGEC），取第三代细胞分为正常组（培养液含5.5 mmol／L D－葡萄糖）、高糖组（培养液含25 mmol／L D－葡萄糖）和甘露醇组（含5.5 mmol／L D－葡萄糖及19.5 mmol／L甘露醇的培养基），采用MTS法检测细胞的增殖情况，流式细胞术检测细胞的生长周期及凋亡情况，划痕实验检测细胞迁移能力。采用重复测量的方差分析进行统计分析。结果高糖组、正常组、甘露醇组三组hGEC相比较，细胞增殖活性（F=5.053，P＝0.052）、细胞周期分布（F=1.252，P＝0.351）及凋亡细胞比例（F=0.325，P＝0.717）差异均无统计学意义；划痕24 h时，糖尿病组的划痕迁移率显著低于正常组（F=54.453， P=0.000）。结论高糖状态下hGEC增殖及凋亡无显著性改变，但细胞迁移能力出现显著下降。
목적：관찰고당상태대인아간상피세포생물학특성적영향。방법원대배양인아간상피세포（hGEC），취제삼대세포분위정상조（배양액함5.5 mmol／L D－포도당）、고당조（배양액함25 mmol／L D－포도당）화감로순조（함5.5 mmol／L D－포도당급19.5 mmol／L감로순적배양기），채용MTS법검측세포적증식정황，류식세포술검측세포적생장주기급조망정황，화흔실험검측세포천이능력。채용중복측량적방차분석진행통계분석。결과고당조、정상조、감로순조삼조hGEC상비교，세포증식활성（F=5.053，P＝0.052）、세포주기분포（F=1.252，P＝0.351）급조망세포비례（F=0.325，P＝0.717）차이균무통계학의의；화흔24 h시，당뇨병조적화흔천이솔현저저우정상조（F=54.453， P=0.000）。결론고당상태하hGEC증식급조망무현저성개변，단세포천이능력출현현저하강。
Objective To investigate the biological characteristics of human gingival epithelial cells ( hGECs ) in high-glucose environments . Methods Cultured primary hGECs , logarithmic growth phase of the 3rd passage cells were used for subsequent experiments . Experimental groups:(1) high glucose group: K-SFM medium containing 25 mmol/L D-glucose;(2) normal group: K-SFM medium containing 5.5 mmol/L D-glucose;(3) mannitol group: K-SFM medium containing 5.5 mmol/L D-glucose and 19.5 mmol/L mannitol. We used the MTS method to detect the proliferation of hGECs, applied flow cytometry to detect cell cycle and apoptosis of hGECs , and utilized wound healing test to detect migration of hGECs. Comparisons of the data among different groups were performed by repeated measures analysis of variance (ANOVA). Results There is no statistically significant difference in cell proliferation activity (F=5.053,P=0.052), proportion of cell proliferate (DNA synthesis phase)(F=1.252,P=0.351) and cell apoptosis (F=0.325,P=0.717). Wound healing test showed after culture for 24 h, the scratch migration rate of hGECs in the high group was significantly lower than cells in the normal glucose group and mannitol group (F=54.453,P=0.000). Conclusion High glucose had no significantly effect on cell morphology, cell proliferation and apoptosis of hGECs. However, it weakened the ability of cell migration of hGECs.