CAJ | 학술논문

目的：探讨 HBV 携带者外周血巨噬细胞移动抑制因子（MIF）、白细胞介素（IL）17和 IL －10水平变化，及其与肝功能水平和 HBV DNA 定量的相关性。方法收集2012年2月－2013年12月于兰州市第二人民医院就诊的60例 HBV 携带者空腹血清样本，其中慢性 HBV 携带者39例，非活动性 HBsAg 携带者21例；采用 ELISA 法检测血清细胞因子 MIF、IL －17和 IL －10水平；采用荧光定量 PCR 法测定各组 HBV DNA 定量，应用全自动生化分析仪检测血清肝功能水平，进行临床相关性分析。同期50例健康体检者为对照组。计量资料组间比较采用配对 t 检验，相关性比较采用 Pearson 相关分析。结果与健康对照组相比，慢性 HBV 携带者组和非活动性 HBsAg 携带者组外周血 MIF、IL －17和 IL －10水平均显著升高，差异有统计学意义（t 值分别为4．50，3．11，9．99；6．28，4．11，14．96；P 值均＜0．01），其中慢性 HBV 携带者组 MIF、IL －17和 IL －10水平低于非活动性 HBsAg 携带者组，但差异无统计学意义（P 值均＞0．05）。慢性 HBV 携带者中中病毒载量组患者 MIF 和 IL －17较其他组低，而 IL －10水平高于另外两组，但差异均无统计学意义（P 值均＞0．05）；HBV 携带者外周血 MIF、IL －17和 IL －10水平与 ALT、AST、TBil、Alb 以及 HBV DNA定量均无相关性（P 值均＞0．05）。结论细胞因子 MIF、IL －17和 IL －10在慢性 HBV 感染过程中可能起重要作用，检测血清细胞因子 MIF、IL －17和 IL －10水平可作为评估 HBV 携带者肝脏炎症严重程度的重要指标。
목적：탐토 HBV 휴대자외주혈거서세포이동억제인자（MIF）、백세포개소（IL）17화 IL －10수평변화，급기여간공능수평화 HBV DNA 정량적상관성。방법수집2012년2월－2013년12월우란주시제이인민의원취진적60례 HBV 휴대자공복혈청양본，기중만성 HBV 휴대자39례，비활동성 HBsAg 휴대자21례；채용 ELISA 법검측혈청세포인자 MIF、IL －17화 IL －10수평；채용형광정량 PCR 법측정각조 HBV DNA 정량，응용전자동생화분석의검측혈청간공능수평，진행림상상관성분석。동기50례건강체검자위대조조。계량자료조간비교채용배대 t 검험，상관성비교채용 Pearson 상관분석。결과여건강대조조상비，만성 HBV 휴대자조화비활동성 HBsAg 휴대자조외주혈 MIF、IL －17화 IL －10수평균현저승고，차이유통계학의의（t 치분별위4．50，3．11，9．99；6．28，4．11，14．96；P 치균＜0．01），기중만성 HBV 휴대자조 MIF、IL －17화 IL －10수평저우비활동성 HBsAg 휴대자조，단차이무통계학의의（P 치균＞0．05）。만성 HBV 휴대자중중병독재량조환자 MIF 화 IL －17교기타조저，이 IL －10수평고우령외량조，단차이균무통계학의의（P 치균＞0．05）；HBV 휴대자외주혈 MIF、IL －17화 IL －10수평여 ALT、AST、TBil、Alb 이급 HBV DNA정량균무상관성（P 치균＞0．05）。결론세포인자 MIF、IL －17화 IL －10재만성 HBV 감염과정중가능기중요작용，검측혈청세포인자 MIF、IL －17화 IL －10수평가작위평고 HBV 휴대자간장염증엄중정도적중요지표。
Objective To determine the changes in peripheral plasma concentrations of macrophage migration inhibitory factor (MIF),in-terleukin -17 (IL -17),and interleukin -10 (IL -10)in HBV carriers and their correlations with liver function or HBV DNA load of HBV carriers.Methods Fasting plasma samples were collected from 60 HBV carriers who were treated at the Second People’s Hospital of Lanzhou from February 2012 to December 2013,including 39 chronic HBV carriers and 21 inactive HBsAg carriers.The plasma concentra-tions of MIF,IL -17,and IL -10 were determined using ELISA.HBV DNA load was measured by quantitative real -time PCR.Liver function was tested using an automatic biochemical analyzer.Fifty healthy subjects served as controls.Continuous data were compared using the paired t test.Correlation analysis was performed using the Pearson correlation test.Results Compared with those in healthy controls, plasma MIF,IL -17,and IL -10 levels in chronic HBV carriers and inactive HBsAg carriers increased significantly (t =4.50,3.11,and 9.99;6.28,4.11,and 14.96;all P <0.01).Plasma MIF,IL -17,and IL -10 levels in chronic HBV carriers were lower than those in inactive HBsAg carriers,though not reaching statistical significance (P >0.05).In chronic HBV carriers with different HBV DNA levels, we found that carriers with medium HBV DNA levels had lower MIF and IL -17 levels,but higher IL -10 levels,though the differences were not statistically significant (P >0.05).There were no correlations between peripheral plasma levels of cytokines (MIF,IL -17,and IL -10)and liver function (ALT,AST,TBil,and Alb)or HBV DNA load (all P >0.05).Conclusion MIF,IL -17,and IL -10 may play an important role in chronic HBV infection,and plasma levels of MIF,IL -17,and IL -10 can be used as important indicators to as-sess the severity of chronic hepatitis B in HBV carriers.