CAJ | 학술논문

目的：探讨重复移植骨髓间充质干细胞(BMSCs)治疗大鼠脑梗死 bcl-2、caspase-3蛋白表达。方法BMSCs 体外培养、纯化。线栓法建立大鼠永久性大脑中动脉阻塞模型,随机分为对照组(n=15)：1,7d 分别注射1ml 生理盐水；单次组(n=15)：1d,注射1ml 细胞悬液,7d 注射1ml 生理盐水；重复组(n=15)在1,7d 注射1ml 细胞悬液,均尾静脉注射。神经功能评分进行行为学评价；TTC 染色评估梗死体积；免疫组化及 western 检测 bcl-2、caspase-3蛋白表达。结果单次组、重复组 mNSS 评分比对照组显著降低(P <0.01),同组比较评分降低(P <0.05)；单次组、重复组相对梗死体积比对照组小(P <0.05),单次组和重复组 bcl-2阳性细胞灰度值比对照组大(P <0.01),caspase-3阳性细胞灰度值降低(P <0.05),单次组和重复组 bcl-2蛋白含量比对照组多(P <0.01),caspase-3蛋白含量降低(P <0.05)。结论重复移植 BMSCs 治疗脑梗死可更好改善大鼠神经功能,减少梗死体积,可能与上调 bcl-2蛋白表达,减少 caspase-3蛋白表达有关系。
목적：탐토중복이식골수간충질간세포(BMSCs)치료대서뇌경사 bcl-2、caspase-3단백표체。방법BMSCs 체외배양、순화。선전법건립대서영구성대뇌중동맥조새모형,수궤분위대조조(n=15)：1,7d 분별주사1ml 생리염수；단차조(n=15)：1d,주사1ml 세포현액,7d 주사1ml 생리염수；중복조(n=15)재1,7d 주사1ml 세포현액,균미정맥주사。신경공능평분진행행위학평개；TTC 염색평고경사체적；면역조화급 western 검측 bcl-2、caspase-3단백표체。결과단차조、중복조 mNSS 평분비대조조현저강저(P <0.01),동조비교평분강저(P <0.05)；단차조、중복조상대경사체적비대조조소(P <0.05),단차조화중복조 bcl-2양성세포회도치비대조조대(P <0.01),caspase-3양성세포회도치강저(P <0.05),단차조화중복조 bcl-2단백함량비대조조다(P <0.01),caspase-3단백함량강저(P <0.05)。결론중복이식 BMSCs 치료뇌경사가경호개선대서신경공능,감소경사체적,가능여상조 bcl-2단백표체,감소 caspase-3단백표체유관계。
Objective To investigate the expression of bcl-2 and caspase-3 protein on the ischemic cere-bral infarction in rat with repeat administered bone marrow mesenchymal stem cells(BMSCs ).Methods In vitro cultivated and purified the BMSCs of the Sprague Dawley rats and established cerebral ischemia model with modified Zea Longa method.Forty-five rats were divided into three groups (control group, single group and repeat group),15 rats in each group,and then treated respectively with intravenous injec-tion of saline 1ml 1d and 7d after MCAO or BMSCs 1ml 1d and saline 1ml 7d or BMSCs 1ml 1d and 7d. Cerebral infarction volume of rats was observed by TTC staining.The expression of bcl-2 and caspase-3 protein was detected through Immunohistochemistry and Western blot.Results Compared with the control group,in the single group and repeat group,the mNSS score was remarkable lower,the cerebral infaration volume was smaller,the expression of caspase-3 protein was also lower,but the expression of bcl-2 was higher(allP <0.05).In addition,the above indicators were better in the repeat group than those in the sin-gle group(allP <0.05).Conclusion Repeat transplantation of BMSCs after stroke in rats can improve the neurological function,decrease the infarction volume and the expression of caspase-3 and increase the ex-pression of bcl-2.