CAJ | 학술논문

目的：分析阿托伐他汀对自发性高血压大鼠心肌细胞P P AR－γ及GLUT－4表达水平的影响，为临床运用提供理论指导。方法：选择20只自发性高血压大鼠，随机分为A组（10只）与B组（10只），同时选择10只健康大鼠作为对照组成C组。给予A组大鼠阿托伐他汀按照50mg? kg－1?d－1的水准灌胃喂养，同时给予B组、C组大鼠以同量的蒸馏水灌胃。每隔1周测量三组大鼠的收缩压与舒张压值，测量5次，直到第10周；同时检测三组大鼠10周平均的血脂水平与左心室质量指数水平，比较三组大鼠血脂水平差异性；采用免疫组化法及PT－PCR法检测三组大鼠的PPAR－γ及GLUT－4表达水平。结果：A组与B组大鼠的舒张压与收缩压水平较C组大鼠均较高，具有明显差异性（P＜0．05），A组与B组大鼠的血压值水平比较，差异性不明显（ P＞0．05）；A组与B组大鼠的TC、TG、HDL－C以及LDL－C等血脂水平较C组大鼠均较低，具有明显差异性（ P＜0．05），A组与B组比较血脂水平无差异性（P＞0．05）。三组大鼠的LVM1水平两两比较均具有显著性差异（P＜0．05），但B组大鼠的LVM1水平最高，其次是A组，C组大鼠水平最低；干预前，A组与B组大鼠PPAR－γ及GLUT－4表达水平均较C组较低，具有统计学意义（ P＜0．05），但A组与B组比较差异性不大（ P＞0．05）；干预后，A组大鼠P P AR－γ及GLUT－4表达水平显著提高，与C组大鼠比较差异性不大，但与B组大鼠比较，具有较大差异性（ P＜0．05）。结论：阿托伐他汀喂养自发性高血压大鼠，能够降低左心室质量指数水平，但对血压、血脂水平无明显影响，这可能与上调P P AR－γ及GLUT－4表达水平有关，具体机制需要进一步研究。
목적：분석아탁벌타정대자발성고혈압대서심기세포P P AR－γ급GLUT－4표체수평적영향，위림상운용제공이론지도。방법：선택20지자발성고혈압대서，수궤분위A조（10지）여B조（10지），동시선택10지건강대서작위대조조성C조。급여A조대서아탁벌타정안조50mg? kg－1?d－1적수준관위위양，동시급여B조、C조대서이동량적증류수관위。매격1주측량삼조대서적수축압여서장압치，측량5차，직도제10주；동시검측삼조대서10주평균적혈지수평여좌심실질량지수수평，비교삼조대서혈지수평차이성；채용면역조화법급PT－PCR법검측삼조대서적PPAR－γ급GLUT－4표체수평。결과：A조여B조대서적서장압여수축압수평교C조대서균교고，구유명현차이성（P＜0．05），A조여B조대서적혈압치수평비교，차이성불명현（ P＞0．05）；A조여B조대서적TC、TG、HDL－C이급LDL－C등혈지수평교C조대서균교저，구유명현차이성（ P＜0．05），A조여B조비교혈지수평무차이성（P＞0．05）。삼조대서적LVM1수평량량비교균구유현저성차이（P＜0．05），단B조대서적LVM1수평최고，기차시A조，C조대서수평최저；간예전，A조여B조대서PPAR－γ급GLUT－4표체수평균교C조교저，구유통계학의의（ P＜0．05），단A조여B조비교차이성불대（ P＞0．05）；간예후，A조대서P P AR－γ급GLUT－4표체수평현저제고，여C조대서비교차이성불대，단여B조대서비교，구유교대차이성（ P＜0．05）。결론：아탁벌타정위양자발성고혈압대서，능구강저좌심실질량지수수평，단대혈압、혈지수평무명현영향，저가능여상조P P AR－γ급GLUT－4표체수평유관，구체궤제수요진일보연구。
Objective:To analyse affect of spontaneously hypertensive rat cardiomyocyte expression of PPAR-γand GLUT-4 with atorvastatin, in order to provide theoretical guidance for clinical application. Method:20 spontaneously hypertensive rats were randomly divided into group A(10) and group B(10), 10 healthy rats were selected as group C.Group A were feeded with atorvastatin in dose of 50mg? kg-1? d-1 by gavaging, group B and group C were feeded with the same amount of distilled water by gavaging.Systolic and diastolic blood pressure values of the three groups were measured every other week until 10 weeks;Lipid lev-els and left ventricular mass index of three groups were measured for 10 weeks, Lipid levels of three groups were compared;PPAR-γand GLUT-4 expression levels of three groups were detected by immunohistochem-istry and PT-PCR method.Result:Diastolic and systolic blood pressure levels in group A and group B rats were higher than group C rats ( P <0.05) , group A and group B median level of blood pressure had no sig-nificant ( P>0.05);lipid levels of TC, TG, HDL-C and LDL-C in group A and group B were lower than group C rats ( P <0.05) , lipid levels had no relatively difference between group A and group B ( P>0.05) . LVM1 levels among three groups were significant differences ( P <0.05), and group B was the highest,group A was Secondly higher,group C was the lowest; before the intervention, PPAR-γand GLUT-4 ex-pression of group A and group B was lower than group C ( P <0.05) , but group A and group B were not sig-nificant ( P>0.05); after the intervention, PPAR-γand GLUT-4 expression levels was significantly in-creased, there was not significant compared with group C, but there was significant different compared with group B ( P <0.05) .Conclusion:Atorvastatin fed spontaneously hypertensive rats, can reduce the level of left ventricular mass index, blood pressure, but no significant effect on blood lipid levels, which may in-crease PPAR-γand GLUT-4 expression levels, the specific mechanisms needed further study.