皮肤病与性病
皮膚病與性病
피부병여성병
JOURNAL OF DERMATOLOGY AND VENEROLOGY
2015年
2期
70-75
,共6页
王芳%林新瑜%刘刚%廖恒利%刘伟%陈明懿%王艺淳
王芳%林新瑜%劉剛%廖恆利%劉偉%陳明懿%王藝淳
왕방%림신유%류강%료항리%류위%진명의%왕예순
黑素细胞%白介素-10%白介素-17
黑素細胞%白介素-10%白介素-17
흑소세포%백개소-10%백개소-17
melanocyte%IL-10%IL-17
目的：观察不同浓度白介素-10，白介素-17对黑素细胞生物活性的影响。方法建立模拟正常生理状态的黑素细胞体外培养体系，用不同浓度白介素-10，白介素-17作用于黑素细胞后，采用CCK-8法测定白介素-10，白介素-17对黑素细胞增殖活性的影响、L-DOPA法测定对黑素细胞酪氨酸酶活性的影响、流式细胞仪检测黑素细胞凋亡率。结果①50μg／ml IL-10组对黑素细胞的增殖率随着培养时间的延长而升高，当培养48h和72h后与空白对照组相比有极显著的统计学差异（ P＜0.01）。但随着IL-10浓度的升高，其增殖率随着培养时间的延长反而有下降趋势，200μg／ml IL-10组培养黑素细胞48h和72h后与空白对照组相比有统计学差异（ P＜0.05）；②IL-17随着培养时间的延长呈剂量依赖性抑制黑素细胞的增殖，50μg／ml IL-17组培养黑素细胞48h和72h后与空白对照组相比有统计学差异（ P＜0.05）；100μg／ml IL-17组和200μg／ml IL-17组培养黑素细胞48h和72h后与空白对照组相比有极显著的统计学差异（ P＜0.01）；③在培养48h后，50μg／ml IL-10组黑素细胞酪氨酸酶活性升高，较空白对照组有统计学差异（ P＜0.05）。50μg／ml、100μg／ml、200μg／ml IL-17组抑制黑素细胞酪氨酸酶的活性，与空白对照组相比有统计学差异（P＜0.05）；④用浓度为100μg／ml的IL-10，IL-17培养黑素细胞，48小时后细胞凋亡率分别为5.2％、32.1％，空白对照组为5.1％。结论①适当浓度的IL-10可促进黑素细胞的增殖，提高酪氨酸酶的活性；② IL-17对黑素细胞有损伤作用，但其作用机制还需进一步的试验和探讨。
目的：觀察不同濃度白介素-10，白介素-17對黑素細胞生物活性的影響。方法建立模擬正常生理狀態的黑素細胞體外培養體繫，用不同濃度白介素-10，白介素-17作用于黑素細胞後，採用CCK-8法測定白介素-10，白介素-17對黑素細胞增殖活性的影響、L-DOPA法測定對黑素細胞酪氨痠酶活性的影響、流式細胞儀檢測黑素細胞凋亡率。結果①50μg／ml IL-10組對黑素細胞的增殖率隨著培養時間的延長而升高，噹培養48h和72h後與空白對照組相比有極顯著的統計學差異（ P＜0.01）。但隨著IL-10濃度的升高，其增殖率隨著培養時間的延長反而有下降趨勢，200μg／ml IL-10組培養黑素細胞48h和72h後與空白對照組相比有統計學差異（ P＜0.05）；②IL-17隨著培養時間的延長呈劑量依賴性抑製黑素細胞的增殖，50μg／ml IL-17組培養黑素細胞48h和72h後與空白對照組相比有統計學差異（ P＜0.05）；100μg／ml IL-17組和200μg／ml IL-17組培養黑素細胞48h和72h後與空白對照組相比有極顯著的統計學差異（ P＜0.01）；③在培養48h後，50μg／ml IL-10組黑素細胞酪氨痠酶活性升高，較空白對照組有統計學差異（ P＜0.05）。50μg／ml、100μg／ml、200μg／ml IL-17組抑製黑素細胞酪氨痠酶的活性，與空白對照組相比有統計學差異（P＜0.05）；④用濃度為100μg／ml的IL-10，IL-17培養黑素細胞，48小時後細胞凋亡率分彆為5.2％、32.1％，空白對照組為5.1％。結論①適噹濃度的IL-10可促進黑素細胞的增殖，提高酪氨痠酶的活性；② IL-17對黑素細胞有損傷作用，但其作用機製還需進一步的試驗和探討。
목적：관찰불동농도백개소-10，백개소-17대흑소세포생물활성적영향。방법건립모의정상생리상태적흑소세포체외배양체계，용불동농도백개소-10，백개소-17작용우흑소세포후，채용CCK-8법측정백개소-10，백개소-17대흑소세포증식활성적영향、L-DOPA법측정대흑소세포락안산매활성적영향、류식세포의검측흑소세포조망솔。결과①50μg／ml IL-10조대흑소세포적증식솔수착배양시간적연장이승고，당배양48h화72h후여공백대조조상비유겁현저적통계학차이（ P＜0.01）。단수착IL-10농도적승고，기증식솔수착배양시간적연장반이유하강추세，200μg／ml IL-10조배양흑소세포48h화72h후여공백대조조상비유통계학차이（ P＜0.05）；②IL-17수착배양시간적연장정제량의뢰성억제흑소세포적증식，50μg／ml IL-17조배양흑소세포48h화72h후여공백대조조상비유통계학차이（ P＜0.05）；100μg／ml IL-17조화200μg／ml IL-17조배양흑소세포48h화72h후여공백대조조상비유겁현저적통계학차이（ P＜0.01）；③재배양48h후，50μg／ml IL-10조흑소세포락안산매활성승고，교공백대조조유통계학차이（ P＜0.05）。50μg／ml、100μg／ml、200μg／ml IL-17조억제흑소세포락안산매적활성，여공백대조조상비유통계학차이（P＜0.05）；④용농도위100μg／ml적IL-10，IL-17배양흑소세포，48소시후세포조망솔분별위5.2％、32.1％，공백대조조위5.1％。결론①괄당농도적IL-10가촉진흑소세포적증식，제고락안산매적활성；② IL-17대흑소세포유손상작용，단기작용궤제환수진일보적시험화탐토。
Objective To observe the effects of IL-10, IL-17 on biological activity of human epidermal melano-cytes.Methods The melanocytes were treated with IL-10 and IL-17 of different concentration.The proliferation and apoptosis of melanocytes, the activity of tyrosinase were analyzed.Results ① With the increased culture time, the proliferation rate of cultured human epidermal melanocytes in the presence of IL-10 at the concentration of 50μg/mL increased.When melanocytes were cultured for 48h and 72h, there were statistically significant differences ( P<0.01) in proliferation rate of cultured human epidermal melanocytes compared with the control group.However, with the higher IL-10 concentration the proliferation rate of melanocytes manifests a downward trend with the prolonged in-cubation time.When melanocytes were cultured for 48h and 72h, the concentration rate of melanocytes in the pres-ence of IL-10 at the concentration of 200ng/ml has significant differences compared with the control group ( P<0.05) .②With the increased incubation time, the inhibitory ratio of melanocytes was increased in a dose-dependent manner in the presence of IL-17.The inhibitory ratio of melanocytes between the IL-17 groups and the control group were significant difference ( P<0.05) at the concentration of 50μg/ml and statistically significant ( P<0.01) at the concentration of 100μg/ml and 200μg/ml for 48h and 72h.③The melanocyte tyrosinase activity of IL-10 groups in-creased and there are significant differences between IL-10 groups and the control group at the concentration of 50μg/ml for 48h ( P<0.05) .The melanocyte tyrosinase activity were inhibited in the IL-17 group, and there were significant differences between the three groups and the control group at the concentration of 50μg/ml, 100μg/ml and 200μg/ml for the IL-17 group.④Melanocytes were cultured at the concentration of 100μg/ml of IL-10, IL-17 and control group for 48h, the apoptosis rate of melanocytes were 5.2%, 32.1%and 5.1%respectivel.Conclusion The appropriate concentration of IL-10 would promote the proliferation of melanocytes and the tyrosinase activity.IL-17 induced injuries on melanocytes, but the mechanisms need further research and discussion.
