CAJ | 학술논문

目的：探讨肺癌患者化疗前后外周血淋巴细胞中多药耐药基因（ MDR－1）和多药耐药相关蛋白（ MRP）的表达及意义。方法分别于化疗开始前及化疗周期结束后第3周收集肺癌患者外周血标本。采用逆转录聚合酶链反应（RT－PCR）法检测39例肺癌患者外周血中MDR－1mRNA和MRP mR-NA的表达情况，并与20例健康体检者进行对比。结果病例组化疗前MDR－1 mRNA和MRP mRNA的阳性表达率与健康对照组相比差异没有统计学意义（P＞0．05）；各种病理类型的肺癌患者外周血MDR－1mRNA和MRP mRNA的阳性表达水平随着化疗次数的增多而增强，其中小细胞肺癌患者化疗后MDR－1 mRNA和MRP mRNA的表达水平均高于化疗前，差异具有统计学意义。结论化疗可诱导肺癌患者外周血淋巴细胞MDR－1mRNA和MRP mRNA的表达水平增强；应用外周血评估MDR－1mRNA和MRP mRNA的方法简单可靠，创伤性小，可能成为预估肺癌患者化疗疗效的有效指标。
목적：탐토폐암환자화료전후외주혈림파세포중다약내약기인（ MDR－1）화다약내약상관단백（ MRP）적표체급의의。방법분별우화료개시전급화료주기결속후제3주수집폐암환자외주혈표본。채용역전록취합매련반응（RT－PCR）법검측39례폐암환자외주혈중MDR－1mRNA화MRP mR-NA적표체정황，병여20례건강체검자진행대비。결과병례조화료전MDR－1 mRNA화MRP mRNA적양성표체솔여건강대조조상비차이몰유통계학의의（P＞0．05）；각충병리류형적폐암환자외주혈MDR－1mRNA화MRP mRNA적양성표체수평수착화료차수적증다이증강，기중소세포폐암환자화료후MDR－1 mRNA화MRP mRNA적표체수평균고우화료전，차이구유통계학의의。결론화료가유도폐암환자외주혈림파세포MDR－1mRNA화MRP mRNA적표체수평증강；응용외주혈평고MDR－1mRNA화MRP mRNA적방법간단가고，창상성소，가능성위예고폐암환자화료료효적유효지표。
Objective To determine the significance of MDR -1 and MRP mRNA expression in periph-eral blood lymphocytes in lung cancer patients with chemotherapy treatment .Methods Peripheral blood samples were taken from each lung cancer patient before chemotherapy and three weeks after the first chemotherapy cycle . The expressions of MDR-1 and MRP were tested for 39 lung cancer cases using RT -PCR technique comparing to 20 normal control cases .Results There was no significant difference for MDR -1 mRNA and MRP mRNA ex-isting in lung cancer cases and control group (P>0.05).MDR-1 and MRP mRNA expressions were increased after treatment of chemotherapy courses in almost all pathological types of lung cancer .Furthermore,the expres-sion level in small cell lung cancer after chemotherapy was significantly higher than that before .Conclusion Chemotherapy may induce the incidence rate of MDR -1mRNA and MRP mRNA expression in lung cancer .Ade-nocarcinoma and squamous cell carcinoma are mainly intrinsic MDR while small cell lung cancer is mainly ac -quired MDR to chemotherapy .Based on the positive expression rate of MDR -1 mRNA and MRP mRNA in pe-ripheral blood ,we may evaluate the results of chemotherapy in lung cancer patients easily and conveniently .