CAJ | 학술논문

目的：探讨大肠癌患者外周血中 CD4+和 CD8+T 淋巴细胞亚群的变化以及与患者预后的关系。方法选取2012年6月—2014年6月到该院诊治的47例大肠癌患者作为研究对象，同时选取同期到该院接受体检的健康者作为对照研究。二组均抽取静脉血，采用流式细胞术检测外周血中 CD4+和 CD8+T 淋巴细胞的比例以及 CD4/CD8比例，并分析了 CD4/CD8比例与患者早期大肠癌患者临床参数以及预后的关系。结果与对照组比较，观察组患者外周血中 CD4+T 淋巴细胞和 CD4/CD8比例均显著下调(P <0.05)，而 CD8+T 淋巴细胞比例则显著提高(P <0.05)。CD4/CD8比例与患者年龄、性别、组织分化程度和 Dukes 分期无关，而与淋巴结转移以及生存时间有关。结论CD4+和 CD8+T 淋巴细胞水平反映了早期大肠癌患者的免疫功能，与淋巴结转移以及预后密切相关。
목적：탐토대장암환자외주혈중 CD4+화 CD8+T 림파세포아군적변화이급여환자예후적관계。방법선취2012년6월—2014년6월도해원진치적47례대장암환자작위연구대상，동시선취동기도해원접수체검적건강자작위대조연구。이조균추취정맥혈，채용류식세포술검측외주혈중 CD4+화 CD8+T 림파세포적비례이급 CD4/CD8비례，병분석료 CD4/CD8비례여환자조기대장암환자림상삼수이급예후적관계。결과여대조조비교，관찰조환자외주혈중 CD4+T 림파세포화 CD4/CD8비례균현저하조(P <0.05)，이 CD8+T 림파세포비례칙현저제고(P <0.05)。CD4/CD8비례여환자년령、성별、조직분화정도화 Dukes 분기무관，이여림파결전이이급생존시간유관。결론CD4+화 CD8+T 림파세포수평반영료조기대장암환자적면역공능，여림파결전이이급예후밀절상관。
Objective To investigate the changes of peripheral blood CD4 and CD8 T lymphocyte subsets and the relationship between T lymphocyte subsets and prognosis.Methods Totally 47 cases of patients with colon cancer from June 2012 to June 2014 in author's hospital were choose as observation group and 47 cases of healthy people were choose as control group.Peripheral blood from two groups was obtained,and CD4+ ,CD8+ T lymphocyte and CD4+/CD8+ ratio were analyzed by flow cytometry.The relationship of CD4+/CD8+ ratio and clinical parameters and prognosis were analyzed.Results Compared with con-trol group,CD4+ T lymphocyte and CD4+/CD8+ ratio in observation group were significantly reduced (P <0.05),while CD8+ T lymphocyte significantly enhanced (P <0.05).There was no relationship be-tween CD4+/CD8+ ratio and patients'age,gender,histological differentiation and Dukes staging.Therer was relationship between CD4+/CD8+ ratio and lymph node metastasis and survival time.Conclusion CD4+ and CD8+T lymphocyte levels reflect the early immune function of patients with colorectal cancer and is closely related to lymph node metastasis and prognosis.