CAJ | 학술논문

采用斑马鱼作为模式动物,针对斑马鱼的代谢能力,进行3种磺胺类药物(磺胺甲恶唑,磺胺嘧啶,磺胺二甲嘧啶)的暴露实验。实验选取谷胱甘肽s-转移酶(GST)和丙二醛(MDA)含量作为评判斑马鱼代谢能力的指标。在实验过程中,随着暴露时间的改变,GST活性和MDA含量都呈现出不同的变化。在实验前3 d,GST酶活性在不同浓度下都呈现出显著增长。但是MDA的含量在第1天达到顶峰,随后都呈现出下降趋势。由结果可得,MDA含量指标对磺胺类药物的毒性更加敏感,其指示作用更优于GST活性指标。同时,实验结果中可以看出,在磺胺嘧啶作用下的GST酶活性和MDA含量都呈现更加明显的变化,因此实验推断磺胺嘧啶会对水环境具有更潜在的危害。
채용반마어작위모식동물,침대반마어적대사능력,진행3충광알류약물(광알갑악서,광알밀정,광알이갑밀정)적폭로실험。실험선취곡광감태s-전이매(GST)화병이철(MDA)함량작위평판반마어대사능력적지표。재실험과정중,수착폭로시간적개변,GST활성화MDA함량도정현출불동적변화。재실험전3 d,GST매활성재불동농도하도정현출현저증장。단시MDA적함량재제1천체도정봉,수후도정현출하강추세。유결과가득,MDA함량지표대광알류약물적독성경가민감,기지시작용경우우GST활성지표。동시,실험결과중가이간출,재광알밀정작용하적GST매활성화MDA함량도정현경가명현적변화,인차실험추단광알밀정회대수배경구유경잠재적위해。
Dispersed pharmaceuticals such as sulfonamides pose a thre at to aquatic ecosystems. We evaluated potential biomarkers during sulfonamide exposure,using an extended zebrafish (Danio rerio),in toxicity test. Glutathione S-transferase (GST) and malondialdehyde (MDA) were selected to reflect the biomarker response of zebrafish exposed to three sulfonamides (sulfamethoxazole, sulfadiazine (SDZ) and sulfadimidine). Both GST and MDA showed time-dependent responses to sulfonamide exposure. GST activity was significantly increased after exposure to sulfonamides for 3 days, while MDA concentration reached a maximum during the first day and then declined. These results suggest that MDA may be a more sensitive biomarker of sulfonamide toxicity than GST. These investigations demonstrated that SDZ was a typical inducer of metabolic enzymes, suggesting that it poses a potential ecotoxicological risk to aquatic ecosystems.