CAJ | 학술논문

以果实口感无酸味的材料60和酸甜味的材料61为亲本构建的含有153株单株的F2群体为试验材料，对其进行SSR标记遗传连锁分析，构建遗传图谱。图谱包含15个连锁群，总长度923.2 cM，平均距离8.71 cM。利用MapQTL 4.0软件采用多座位QTL模型（MQM）进行QTL分析。分别检测到3个与柠檬酸含量有关的QTL，其中1个主效QTL（贡献率≥10%）为cit 8.1，贡献率为34.8%；4个与可滴定酸（TA）有关的QTL，其中1个主效QTL为ta 8.1，贡献率为47.5%；2个与pH有关的QTL，其中1个主效QTL为ph 8.1，贡献率为82.7%。与酸性性状有关的3个主效QTLs均位于第Ⅷ条连锁群上同一位置。
이과실구감무산미적재료60화산첨미적재료61위친본구건적함유153주단주적F2군체위시험재료，대기진행SSR표기유전련쇄분석，구건유전도보。도보포함15개련쇄군，총장도923.2 cM，평균거리8.71 cM。이용MapQTL 4.0연건채용다좌위QTL모형（MQM）진행QTL분석。분별검측도3개여저몽산함량유관적QTL，기중1개주효QTL（공헌솔≥10%）위cit 8.1，공헌솔위34.8%；4개여가적정산（TA）유관적QTL，기중1개주효QTL위ta 8.1，공헌솔위47.5%；2개여pH유관적QTL，기중1개주효QTL위ph 8.1，공헌솔위82.7%。여산성성상유관적3개주효QTLs균위우제Ⅷ조련쇄군상동일위치。
A F2 population including 153 individuals derived from a cross between 2 cultivars ‘60’ and ‘61’,which were acidless and acid respectively,was used to analyze a linkage of SSR markers and construct a genetic map. The map contains 15 linkage groups spanning 923.2 cM with an average of 8.71 cM between markers. The multiple QTL model(MQM)method of software package MapQTL version 4.0 was used to map and analyze QTLs. 3 QTLs were detected for citric acid content,including one major QTL(expl≥10%) namedcit 8.1,which explained 34.8% of the phenotypic variation. 4 QTLs were detected for TA,among which one major QTL named ta 8.1,which explained 47.5% of the phenotypic variation. 2 QTLs were detected for pH,among which one major QTL namedph 8.1,which explained 82.7% of the phenotypic variation. These 3 major QTLs related with acdic properties were co-located on LGⅧ.