CAJ | 학술논문

目的：探讨大鼠局灶性脑挫伤后脑中p35和p25表达变化的时间规律性，为脑损伤时间推断提供更多依据。方法50只成年雄性SD大鼠随机分为损伤即刻（0 h）组，伤后6 h、12 h、24 h、3 d、5 d、7 d、10 d组，同时设立正常组和假手术组，每组5只，建立大鼠局灶性脑挫伤模型，运用HE、免疫组织化学染色及Western印迹法等方法检测损伤不同时间后损伤周边区脑组织p35及p25的蛋白表达。结果正常组和假手术组蛋白表达较多p35和少量p25。在局部脑挫伤后，p35随时间呈现单峰变化，p25随时间呈现双峰变化。结论大鼠局灶性脑挫伤后p35和p25的表达呈现不同规律性并有较好的时间相关性，可为脑损伤时间推断提供较好的依据。
목적：탐토대서국조성뇌좌상후뇌중p35화p25표체변화적시간규률성，위뇌손상시간추단제공경다의거。방법50지성년웅성SD대서수궤분위손상즉각（0 h）조，상후6 h、12 h、24 h、3 d、5 d、7 d、10 d조，동시설립정상조화가수술조，매조5지，건립대서국조성뇌좌상모형，운용HE、면역조직화학염색급Western인적법등방법검측손상불동시간후손상주변구뇌조직p35급p25적단백표체。결과정상조화가수술조단백표체교다p35화소량p25。재국부뇌좌상후，p35수시간정현단봉변화，p25수시간정현쌍봉변화。결론대서국조성뇌좌상후p35화p25적표체정현불동규률성병유교호적시간상관성，가위뇌손상시간추단제공교호적의거。
Objective To study the expression of p35 and p25 in rat after focal cerebral contusion and to provide experimental data for estimating brain injury time. Methods Fifty adult male SD rats were randomly divided into 0 h, 6 h, 12 h, 24 h, 3 d, 5 d, 7 d, 10 d after focal cerebral contusion, control and sham-operated groups (5 rats each group). The focal cerebral contusion rat model was established. The expression of p35 and p25 protein of the damage peripheral zone in brain were detected by HEstain-ing, immunohistochemistry and western blotting at different injury time. Results Alarge number of p35 protein and a small amount of p25 protein were expressed in control group and sham-operated group. After focal cerebral contusion, p35 presented unimodal change with time and p25 presented bimodal changes with time. Conclusion Expression of p35 and p25 showed different regularity with good time correlation, which could help to estimate the brain injury time.