CAJ | 학술논문

目的：研究大鼠双后肢挤压伤后肺、肝细胞的凋亡过程，探讨挤压伤损伤机制。方法建立大鼠双后肢挤压伤模型，采用TUNEL法对大鼠肺、肝细胞凋亡进行检测，免疫组织化学法检测凋亡相关蛋白Bax、Bcl-2、caspase-3的表达。结果与对照组相比，损伤组大鼠双后肢局部肌肉组织明显损伤，肺、肝细胞凋亡明显增多（P<0.05），凋亡相关蛋白Bax上调、Bcl-2下调、caspase-3被激活（P<0.05）。结论大鼠双后肢挤压伤后引起肺、肝细胞凋亡明显增多，可能是损伤释放的相关因子介导了细胞凋亡的发生。
목적：연구대서쌍후지제압상후폐、간세포적조망과정，탐토제압상손상궤제。방법건립대서쌍후지제압상모형，채용TUNEL법대대서폐、간세포조망진행검측，면역조직화학법검측조망상관단백Bax、Bcl-2、caspase-3적표체。결과여대조조상비，손상조대서쌍후지국부기육조직명현손상，폐、간세포조망명현증다（P<0.05），조망상관단백Bax상조、Bcl-2하조、caspase-3피격활（P<0.05）。결론대서쌍후지제압상후인기폐、간세포조망명현증다，가능시손상석방적상관인자개도료세포조망적발생。
Objective To investigate the process of apoptosis in lungs and liver induced by crushing hindlimbs of rat, and study the mechanismof crush injury. Methods The rat experimental model of hindlimbs crush injury was established. The cell apoptosis in lungs and liver was detected by TUNEL assay, and the expression of Bax, Bcl-2 and caspase-3 apoptin was examined by immunohistochemistry. Results C ompared with the control group, the partial muscle injury of rat’s hindlimbs was more serious with more apoptosis observed in lungs and liver (P<0.05). The expression of Bax was up-regulated and Bcl-2 was down-regulated, whereas caspase-3 expression was activated (P<0.05). Conclusion The cell apoptosis has increased significantly in lungs and liver after crush injury of hindlimbs in rat. The corre-lation factor released during tissue injury may mediate apoptosis process.