CAJ | 학술논문

针对高浓度间甲酚废水难降解的问题，通过筛选获得一株高效降解间甲酚的菌株，并进一步探究其对间甲酚的降解特性和降解动力学。本文从某焦化厂活性污泥中经过驯化、筛选分离得到一株以间甲酚为唯一碳源的高效降解菌株，对其进行16S rDNA分子鉴定；通过测定培养液中间甲酚的剩余浓度，考察在菌株对间甲酚的最佳降解条件；并采用 Haldane 模型来模拟菌株降解间甲酚的动力学行为。将分离得到的菌株命名为 SMC，初步鉴定为Bacillus cereus；在pH值为7.5、温度为30℃、摇床转速为150r/min的最佳培养条件下，菌株SMC在48h内对浓度1600mg/L的间甲酚降解率达97.81%，对间甲酚最大耐受能力为1900mg/L；经过Haldane模型拟合后的动力学参数为μmax=0.01252h?1，KS=34.58mg/L，Ki=479.5mg/L（R2=0.932）。菌株SMC对间甲酚有较强的降解能力，在高效处理含酚废水方面具有一定的应用前景。
침대고농도간갑분폐수난강해적문제，통과사선획득일주고효강해간갑분적균주，병진일보탐구기대간갑분적강해특성화강해동역학。본문종모초화엄활성오니중경과순화、사선분리득도일주이간갑분위유일탄원적고효강해균주，대기진행16S rDNA분자감정；통과측정배양액중간갑분적잉여농도，고찰재균주대간갑분적최가강해조건；병채용 Haldane 모형래모의균주강해간갑분적동역학행위。장분리득도적균주명명위 SMC，초보감정위Bacillus cereus；재pH치위7.5、온도위30℃、요상전속위150r/min적최가배양조건하，균주SMC재48h내대농도1600mg/L적간갑분강해솔체97.81%，대간갑분최대내수능력위1900mg/L；경과Haldane모형의합후적동역학삼수위μmax=0.01252h?1，KS=34.58mg/L，Ki=479.5mg/L（R2=0.932）。균주SMC대간갑분유교강적강해능력，재고효처리함분폐수방면구유일정적응용전경。
A bacterial strain SMC was isolated from the aerobic sludge of coking wastewater to resolve the problem of difficulty in treating effluent with high concentration ofm-cresol. The characteristics and kinetics of degradation of m-cresol by the strain was investigated withm-cresol as sole source of carbon in the cultural medium. After isolation and screening,16S rDNA gene was analyzed to identify the taxonomic position of the strain. Its degradation capability under optimal growth conditions was studied by testing the concentration of m-cresol in residue medium. Haldane type substrate inhibit model was used to simulate cell growth onm-cresol. The isolated strain named as SMC belonged to Bacillus cereus. Under the optimal conditions of pH 7.5、30℃,150r/min of shaking rate,m-cresol with 1600mg/L initial concentration would be degraded 97.81% in 48h and the highest tolerance tom-cresol was 1900mg/L. The kinetic parameters were:μmax=0.01252h?1,KS=34.58mg/L,Ki=479.5mg/L(R2 = 0.932). SMC could degradem-cresol effectively,with good application potential for wastewater containing high concentration ofm-cresol.