CAJ | 학술논문

目的：探讨单克隆形态结合SEMA3 B检测分离胃癌中的致瘤成分。方法采用克隆的形态并分类，计算克隆形成率；采用Western blot 法检测不同克隆细胞中SEMA3B的表达，并观察各组克隆细胞的体内成瘤能力。结果 SGC－7901细胞可形成3种克隆形态，分为Holoclone 型、Paraclone型和Meroclone型，总克隆形成率为（10．20±1．07）％；Western blot法检测发现SEMA3B在Holoclone型中表达最强，并且Holoclone型克隆细胞具有很强的体内成瘤能力。结论本研究为探寻SEMA3B在胃癌中成瘤和增殖的作用提供了理论依据。
목적：탐토단극륭형태결합SEMA3 B검측분리위암중적치류성분。방법채용극륭적형태병분류，계산극륭형성솔；채용Western blot 법검측불동극륭세포중SEMA3B적표체，병관찰각조극륭세포적체내성류능력。결과 SGC－7901세포가형성3충극륭형태，분위Holoclone 형、Paraclone형화Meroclone형，총극륭형성솔위（10．20±1．07）％；Western blot법검측발현SEMA3B재Holoclone형중표체최강，병차Holoclone형극륭세포구유흔강적체내성류능력。결론본연구위탐심SEMA3B재위암중성류화증식적작용제공료이론의거。
Objce tive To investgate the morphological monoclonal combined with SEMA 3B for detection of the tumorigenic components in gastric cancer .Methods Clones derived from gastric cancer SGC -7901 cells were assessed by morphological observation ,the clone formation rate was calculated .The expression of SEMA3B was detected by Western blot ,and the tumorigenic ability of each group was determined .Results Clones derived from GC SGC-7901 cells had three types,the total clone formation rate was(10.20 ±1.07)%,the expression of SEMA3B was the strongest in the Holoclone colonies ,SGC-7901 cells of Holoclone clones possessed strong abil-ity of self-renewal and in vivo tumorigenicity in the nude mice .Conclusion This study provides the experimen-tal basis for exploring the effect of SEMA 3B in gastric carcinoma tumor formation and proliferation .