CAJ | 학술논문

目的：建立人多瘤病毒7（HPyV7）核酸快速、特异的TaqMan探针实时定量PCR检测方法。方法：分别设计HPyV7特异性的引物与TaqMan探针，建立实时荧光定量PCR方法，并对其特异性、灵敏性和重复性进行评价；用建立的方法检测200份健康成人志愿者的血清和300份急性呼吸道感染住院儿童的鼻咽抽吸物样本。结果：所建立的实时定量PCR方法对HPyV7的检测灵敏度可达10拷贝/μL，检测线性范围为101～1010拷贝/μL，且实验特异性和重复性好（CV<1.0%）；采用该方法，从200份血清样本中检出9份阳性标本。结论：建立了HPyV7 TaqMan探针实时定量PCR检测方法，为HPyV7的流行病学调查及其初步研究提供了技术手段。
목적：건립인다류병독7（HPyV7）핵산쾌속、특이적TaqMan탐침실시정량PCR검측방법。방법：분별설계HPyV7특이성적인물여TaqMan탐침，건립실시형광정량PCR방법，병대기특이성、령민성화중복성진행평개；용건립적방법검측200빈건강성인지원자적혈청화300빈급성호흡도감염주원인동적비인추흡물양본。결과：소건립적실시정량PCR방법대HPyV7적검측령민도가체10고패/μL，검측선성범위위101～1010고패/μL，차실험특이성화중복성호（CV<1.0%）；채용해방법，종200빈혈청양본중검출9빈양성표본。결론：건립료HPyV7 TaqMan탐침실시정량PCR검측방법，위HPyV7적류행병학조사급기초보연구제공료기술수단。
Objective: To develop a rapid and sensitive TaqMan based, real-time quantitation PCR(qPCR) assay for detection and quantitation of huamn polyomavirus 7(HPyV7). Methods: The specific primers and TaqMan probe to develop a real-time qPCR assay for detection of HPyV7 were designed. The specificity, sensitivity and re?producibility of real-time qPCR were evaluated. Subsequently, real-time qPCR was used to identify HPyV7 in se?ra samples of 200 healthy adult volunteers and 300 nasopharyngeal aspirates of children with related pathogens of acute respiratory tract infections. Results: The analytical detection limit of this real-time qPCR assay was 10 cop?ies/μL. This assay allowed quantitation of HPyV7 over span ranging from 101~1010 copies/μL, and showed good specificity and repeatability(CV<1.0%). A total of 200 sera specimens were screened for the presence of HPyV7 by using real-time qPCR and identified 9 specimens positive for HPyV7. Conclusion: A real-time qPCR assay for detection and quantitation of HPyV7 has been developed. This assay provides technical approach for an epide?miological investigation and preliminary study of HPyV7.