现代药物与临床
現代藥物與臨床
현대약물여림상
Drugs & Clinic
2015年
4期
366-369
,共4页
高源%邢亚超%郭俐含%李艳然%倪慧%卿德刚%李宁
高源%邢亞超%郭俐含%李豔然%倪慧%卿德剛%李寧
고원%형아초%곽리함%리염연%예혜%경덕강%리저
新疆阿魏树脂%人肺癌细胞%倍半萜香豆素%farnesiferol C%抑制作用
新疆阿魏樹脂%人肺癌細胞%倍半萜香豆素%farnesiferol C%抑製作用
신강아위수지%인폐암세포%배반첩향두소%farnesiferol C%억제작용
Ferula sinkiangensis resin%human lung cancer cells%sesquiterpene coumarins%farnesiferol C%inhibitory action
目的：研究新疆阿魏的提取物、不同极性部位以及倍半萜香豆素类化合物对人肺癌细胞的体外抑制作用，明确新疆阿魏树脂的活性成分。方法应用MTT法检测新疆阿魏树脂提取物（100 mg/mL）、不同极性部位（100 mg/mL）和倍半萜香豆素化合物（100、50、10、1、0.1μmol/L）对人肺癌细胞系A549、H292、H1792、H460作用48 h后生长的抑制作用，采用抑制率和IC50为指标衡量其活性。结果新疆阿魏树脂氯仿提取物在100 mg/mL质量浓度下对A549、H292、H1792、H460细胞系的抑制率分别为75.45%、65.39%、46.54%、73.68。低极性部位在100 mg/mL质量浓度下对4种细胞系的生长抑制率分别为88.74%、80.62%、60.11%、92.35%。从低极性部位中分离得到的主要倍半萜香豆素类化合物farnesiferol B、farnesiferone A、farnesiferol C、多花素宁，其中farnesiferol C、多花素宁的IC50分别为35.49、15.44、44.25、21.63μmol/L和23.60、17.90、38.33、12.35μmol/L，表现出显著的生长抑制作用，且具有剂量相关性。结论新疆阿魏树脂的低极性部位为体外抑制人肺癌细胞生长作用的活性部位，其所含有的高含量倍半萜香豆素化合物farnesiferol C为其主要活性成分。
目的：研究新疆阿魏的提取物、不同極性部位以及倍半萜香豆素類化閤物對人肺癌細胞的體外抑製作用，明確新疆阿魏樹脂的活性成分。方法應用MTT法檢測新疆阿魏樹脂提取物（100 mg/mL）、不同極性部位（100 mg/mL）和倍半萜香豆素化閤物（100、50、10、1、0.1μmol/L）對人肺癌細胞繫A549、H292、H1792、H460作用48 h後生長的抑製作用，採用抑製率和IC50為指標衡量其活性。結果新疆阿魏樹脂氯倣提取物在100 mg/mL質量濃度下對A549、H292、H1792、H460細胞繫的抑製率分彆為75.45%、65.39%、46.54%、73.68。低極性部位在100 mg/mL質量濃度下對4種細胞繫的生長抑製率分彆為88.74%、80.62%、60.11%、92.35%。從低極性部位中分離得到的主要倍半萜香豆素類化閤物farnesiferol B、farnesiferone A、farnesiferol C、多花素寧，其中farnesiferol C、多花素寧的IC50分彆為35.49、15.44、44.25、21.63μmol/L和23.60、17.90、38.33、12.35μmol/L，錶現齣顯著的生長抑製作用，且具有劑量相關性。結論新疆阿魏樹脂的低極性部位為體外抑製人肺癌細胞生長作用的活性部位，其所含有的高含量倍半萜香豆素化閤物farnesiferol C為其主要活性成分。
목적：연구신강아위적제취물、불동겁성부위이급배반첩향두소류화합물대인폐암세포적체외억제작용，명학신강아위수지적활성성분。방법응용MTT법검측신강아위수지제취물（100 mg/mL）、불동겁성부위（100 mg/mL）화배반첩향두소화합물（100、50、10、1、0.1μmol/L）대인폐암세포계A549、H292、H1792、H460작용48 h후생장적억제작용，채용억제솔화IC50위지표형량기활성。결과신강아위수지록방제취물재100 mg/mL질량농도하대A549、H292、H1792、H460세포계적억제솔분별위75.45%、65.39%、46.54%、73.68。저겁성부위재100 mg/mL질량농도하대4충세포계적생장억제솔분별위88.74%、80.62%、60.11%、92.35%。종저겁성부위중분리득도적주요배반첩향두소류화합물farnesiferol B、farnesiferone A、farnesiferol C、다화소저，기중farnesiferol C、다화소저적IC50분별위35.49、15.44、44.25、21.63μmol/L화23.60、17.90、38.33、12.35μmol/L，표현출현저적생장억제작용，차구유제량상관성。결론신강아위수지적저겁성부위위체외억제인폐암세포생장작용적활성부위，기소함유적고함량배반첩향두소화합물farnesiferol C위기주요활성성분。
Objective To study inhibitory actionin vitro on human lung cancer cells of extract, different fractions, and sesquiterpene coumarins fromFerula sinkiangensis resin, and to clarify its effective constituent.Methods Using MTT method, inhibitory actions of chloroform extract (at concentration of 100 mg/mL), different polarity fractions (at concentration of 100 mg/mL), and sesquiterpene coumarins (at different concentrations of 100, 50, 10, 1, and 0.1μmol/L) fromF. sinkiangensis resin were tested on A549, H292, H1792, and H460 cell lines after 48 h co-incubation. The inhibition rate and IC50values were calculated to assay their effects.Results The chloroform extract fromF. sinkiangensis resin exhibited significant inhibitory action at 100 mg/mL against A549, H292, H1792, and H460 human lung cancer cells with inhibition rates as 75.45%, 65.39%, 46.54%, and 73.68%, respectively. And the low polarity fraction had inhibitory action at 100 mg/mL against the four human lung cancer cells with inhibition rates as 88.74%, 80.62%, 60.11%, and 92.35%, respectively. Sesquiterpene coumarins farnesiferol B, farnesiferone A, farnesiferol C, and polyanthinin were isolated from low polarity fraction, and farnesiferol C and polyanthinin showed significant inhibitory action on growth of human lung cancer cells with dose dependent manner. The IC50values of farnesiferol C were 35.49, 15.44, 44.25, and 21.63 μmol/L, and those of polyanthinin were 23.60, 17.90, 38.33, and 12.35 μmol/L.Conclusion Low polarity fraction is considered as the effective part ofF. sinkiangensis resin for inhibitory activities against human lung cancer cell lines, and the sesquiterpene coumarin farnesiferol C isolated from it is main active constituent.
