CAJ | 학술논문

目的：探讨桂枝茯苓胶囊对子宫肌瘤大鼠的治疗作用及其机制。方法采用注射苯甲酸雌二醇和黄体酮复制大鼠子宫肌瘤模型，造模大鼠按体质量随机分为模型组、他莫昔芬（枸橼酸他莫昔芬片3.6 mg/kg）组以及桂枝茯苓胶囊0.14、0.27、0.54 g/kg组，另设对照组。连续给药8周后处死大鼠，取子宫、脾脏、胸腺并计算其脏器系数，比较子宫病理评分和组织形态，放射免疫分析方法检测血清中雌二醇（E2）、孕酮（P）及子宫组织中表皮生长因子（EGF）水平。结果桂枝茯苓胶囊显著降低大鼠子宫系数，减轻子宫壁平滑肌增生，显著降低血清E2和子宫组织EGF水平。结论桂枝茯苓胶囊对大鼠子宫肌瘤具有治疗作用，其作用机制可能与降低血清E2、子宫组织EGF水平有关。
목적：탐토계지복령효낭대자궁기류대서적치료작용급기궤제。방법채용주사분갑산자이순화황체동복제대서자궁기류모형，조모대서안체질량수궤분위모형조、타막석분（구연산타막석분편3.6 mg/kg）조이급계지복령효낭0.14、0.27、0.54 g/kg조，령설대조조。련속급약8주후처사대서，취자궁、비장、흉선병계산기장기계수，비교자궁병리평분화조직형태，방사면역분석방법검측혈청중자이순（E2）、잉동（P）급자궁조직중표피생장인자（EGF）수평。결과계지복령효낭현저강저대서자궁계수，감경자궁벽평활기증생，현저강저혈청E2화자궁조직EGF수평。결론계지복령효낭대대서자궁기류구유치료작용，기작용궤제가능여강저혈청E2、자궁조직EGF수평유관。
ObjectiveTo discuss the therapeutical effect of Guizhi Fuling Capsule on uterine leiomyoma in rats and its mechanism. MethodsUterine leiomyoma model in rats was made by injection estradiol benzoate and progesterone. The rats with uterine leiomyoma were randomly divided into model group, tamoxifen (Tamoxifen Citrate Tablets 3.6 mg/kg) group, and Guizhi Fuling Capsule (0.14, 0.27, and 0.54 g/kg) groups. There was also a control group. After treatment for eight weeks, the rats were killed by cervical dislocation. The organ coefficients of the uterus, thymus, and spleen were calculated. Pathological score and pathological changes of uterus tissue were detected. The levels of estradiol (E2), progesterone (P) in serum and epidermal growth factor (EGF) in uterus tissue serum were determined by radioimmunoassay.ResultsAfter treatment, Guizhi Fuling Capsule significantly reduced the uterus coefficient, alleviated hyperplasia of smooth muscle in uterus wall, significantly reduced the levels of E2 in serum and EGF in uterine tissue of rats.ConclusionGuizhi Fuling Capsule has therapeutical effect on uterine leiomyoma in rat, and the mechanism may be associated with reduction of E2 levels in serum and EGF levels in uterine tissue.