现代药物与临床
現代藥物與臨床
현대약물여림상
Drugs & Clinic
2015年
4期
380-385
,共6页
马征%杨智%胡春生%马晓宁
馬徵%楊智%鬍春生%馬曉寧
마정%양지%호춘생%마효저
毛叶疏花蔷薇果%提取物%大鼠%亚慢性毒性
毛葉疏花薔薇果%提取物%大鼠%亞慢性毒性
모협소화장미과%제취물%대서%아만성독성
Rosa laxa Retz. var. mollisYu et Ku cynarrhodion%extracts%rats%subchronic toxicity
目的：考察毛叶疏花蔷薇果提取物的亚慢性毒性。方法选用96只健康SD大鼠随机分为4组，雌雄各半，分别ig给予0、1.25、2.50、5.00 g/kg毛叶疏花蔷薇果提取物，连续90 d，观察动物生长情况。试验中、末期对动物的血液学和血清生化指标进行检测，试验末计算动物主要脏器的脏器系数，并进行组织病理学检查。结果试验期间动物无死亡。试验末各剂量组动物体质量增长值、进食量、食物利用率、脏器质量、脏器系数与对照组比较差异均无统计学意义。在试验中、末期各剂量组动物血液学检测指标与对照组比较差异无统计学意义。血清生化检测发现，试验中期5.00 g/kg剂量组雌雄大鼠血清中的肌酐（CR）和雌性大鼠血清中的尿素氮（BUN）水平显著升高；末期5.00 g/kg剂量组雄性大鼠血清中的CR水平仍居高不下，与对照组比较差异均有统计学意义（P＜0.05、0.01），而其他指标均在正常值范围内。病理组织学检查发现5.00 g/kg剂量组部分动物肾小管和小肠可见相关病理改变。结论在本实验条件下，5.00 g/kg毛叶疏花蔷薇果提取物对SD大鼠的肾和小肠有损伤作用，最大无作用剂量为2.50 g/kg。
目的：攷察毛葉疏花薔薇果提取物的亞慢性毒性。方法選用96隻健康SD大鼠隨機分為4組，雌雄各半，分彆ig給予0、1.25、2.50、5.00 g/kg毛葉疏花薔薇果提取物，連續90 d，觀察動物生長情況。試驗中、末期對動物的血液學和血清生化指標進行檢測，試驗末計算動物主要髒器的髒器繫數，併進行組織病理學檢查。結果試驗期間動物無死亡。試驗末各劑量組動物體質量增長值、進食量、食物利用率、髒器質量、髒器繫數與對照組比較差異均無統計學意義。在試驗中、末期各劑量組動物血液學檢測指標與對照組比較差異無統計學意義。血清生化檢測髮現，試驗中期5.00 g/kg劑量組雌雄大鼠血清中的肌酐（CR）和雌性大鼠血清中的尿素氮（BUN）水平顯著升高；末期5.00 g/kg劑量組雄性大鼠血清中的CR水平仍居高不下，與對照組比較差異均有統計學意義（P＜0.05、0.01），而其他指標均在正常值範圍內。病理組織學檢查髮現5.00 g/kg劑量組部分動物腎小管和小腸可見相關病理改變。結論在本實驗條件下，5.00 g/kg毛葉疏花薔薇果提取物對SD大鼠的腎和小腸有損傷作用，最大無作用劑量為2.50 g/kg。
목적：고찰모협소화장미과제취물적아만성독성。방법선용96지건강SD대서수궤분위4조，자웅각반，분별ig급여0、1.25、2.50、5.00 g/kg모협소화장미과제취물，련속90 d，관찰동물생장정황。시험중、말기대동물적혈액학화혈청생화지표진행검측，시험말계산동물주요장기적장기계수，병진행조직병이학검사。결과시험기간동물무사망。시험말각제량조동물체질량증장치、진식량、식물이용솔、장기질량、장기계수여대조조비교차이균무통계학의의。재시험중、말기각제량조동물혈액학검측지표여대조조비교차이무통계학의의。혈청생화검측발현，시험중기5.00 g/kg제량조자웅대서혈청중적기항（CR）화자성대서혈청중적뇨소담（BUN）수평현저승고；말기5.00 g/kg제량조웅성대서혈청중적CR수평잉거고불하，여대조조비교차이균유통계학의의（P＜0.05、0.01），이기타지표균재정상치범위내。병리조직학검사발현5.00 g/kg제량조부분동물신소관화소장가견상관병리개변。결론재본실험조건하，5.00 g/kg모협소화장미과제취물대SD대서적신화소장유손상작용，최대무작용제량위2.50 g/kg。
ObjectiveTo study the subchronic toxicity ofRosa laxa var.mollis fruit extract.Methods SD rats (96) were divided randomly into four groups, and each group consisted of 12 males and 12 females. The animals in four groups were ig administered with R. laxa var.mollis fruit extract at doses of 0 (control), 1.25, 2.50, 5.00 g/kg for 90 d, respectively. During the entire course of the study, the rats were observed daily. The hematological and serum biochemical examinations were conducted after 16 h of starvation at 45th day as an intermittent study and 90th day as a terminal. All animals were calculated organ coefficients and studied for histological changes.ResultsAt the end of the study, no deaths were observed in all groups. There were no differences in body weight gains, food intakes, food availability, and organ coefficients compared to the control (P> 0.05). For the hematological analyses, no parameters demonstrated significant changes in all groups during the entire study. During the serum biochemical analyses, there were significant increases of creatinine (CR) level in 5.00 g/kg both sexes and blood urea nitrogen (BUN) level in 5.00 g/kg female rats at the 45th day. Thereafter, significant increase of CR level in 5.00 g/kg male rats were observed at the end of the study (P< 0.05, 0.01), but the other indexes were in the normal range. In 5.00 g/kg dose group, remarkable histopathological changes were observed in kidney and intestine of the SD rats.ConclusionR. laxa var.mollis fruit extract could be harmful to kidneys and intestines of the SD rats at 5.00 g/kg dose. No observed adverse effect level (NOAEL) is 2.50 g/kg.
