农技服务
農技服務
농기복무
SERVES OF AGRICULTURAL TECHNOLOGY
2015年
3期
55-57
,共3页
朱旭伟%赵学%张爽%张璞瑜%林海萍
硃旭偉%趙學%張爽%張璞瑜%林海萍
주욱위%조학%장상%장박유%림해평
蛹虫草%多糖含量检测%样品前处理%正交试验
蛹蟲草%多糖含量檢測%樣品前處理%正交試驗
용충초%다당함량검측%양품전처리%정교시험
Cordyceps militaris Polysaccharide content%detection%sample pretreatment%orthogonal test
目的:建立蛹虫草多糖含量测定样品前处理方法。方法:以蛹虫草多糖含量为指标,筛选粉碎细度、醇沉时样品浓度、提取温度、提取时间为有关因素,不考虑交互作用,选择三水平进行了L9(34)的正交试验。结果:经极差分析,对多糖检测结果的影响大小的顺序为粉碎细度因素>醇沉时样品浓度>提取时间>提取温度。结论:最佳前处理条件为A3B3C3D2,即为粉碎细度200目、醇沉时样品浓度1g→10ml、提取温度100℃、提取时间1h。
目的:建立蛹蟲草多糖含量測定樣品前處理方法。方法:以蛹蟲草多糖含量為指標,篩選粉碎細度、醇沉時樣品濃度、提取溫度、提取時間為有關因素,不攷慮交互作用,選擇三水平進行瞭L9(34)的正交試驗。結果:經極差分析,對多糖檢測結果的影響大小的順序為粉碎細度因素>醇沉時樣品濃度>提取時間>提取溫度。結論:最佳前處理條件為A3B3C3D2,即為粉碎細度200目、醇沉時樣品濃度1g→10ml、提取溫度100℃、提取時間1h。
목적:건립용충초다당함량측정양품전처리방법。방법:이용충초다당함량위지표,사선분쇄세도、순침시양품농도、제취온도、제취시간위유관인소,불고필교호작용,선택삼수평진행료L9(34)적정교시험。결과:경겁차분석,대다당검측결과적영향대소적순서위분쇄세도인소>순침시양품농도>제취시간>제취온도。결론:최가전처리조건위A3B3C3D2,즉위분쇄세도200목、순침시양품농도1g→10ml、제취온도100℃、제취시간1h。
Objective: to establish a method for determination of sample pretreatment of Cordyceps polysaccharide content. Methods:the cordyceps polysaccharide content as the index selection smash, extraction temperature, fineness, alcohol precipitation concentration samples, extraction time related factors, do not consider the interaction, choose three levels were L9 (34) orthogonal test. Results:after the analysis of range, the influence on the results of polysaccharide detection size order of grinding fineness factor, alcohol precipitation concentration samples > extraction time > extraction temperature. Conclusion: the best processing conditions for A3B3C3D2, namely the crushing fineness of 200 mesh, the alcohol precipitation concentration samples 1g→10ml, extraction temperature 100℃, extraction time 1h.