CAJ | 학술논문

基因表达调控是水稻化感作用形成的重要基础。本研究对化感水稻PI312777(Oryza sativa L.)的OsMYB (CT829537)基因分别进行过表达(overexpression, OE)和RNA干扰(RNAi),再与稗草(Echinochloa crusgalli, BYG)共培养,以野生型PI312777为对照。结果发现,与稗草共培养下CT829537-OEPI312777的酚类代谢关键酶基因表达上调,根系及其水培液中的总酚酸浓度增加,抑草能力增强；相同处理下 CT829537-RNAiPI312777则相反,其酚类代谢关键酶基因较野生型植株表达下调,总酚酸浓度降低,抑草能力下降。这表明OsMYB (CT829537)通过调节化感水稻的酚酸类物质合成进而影响其抑草能力。
기인표체조공시수도화감작용형성적중요기출。본연구대화감수도PI312777(Oryza sativa L.)적OsMYB (CT829537)기인분별진행과표체(overexpression, OE)화RNA간우(RNAi),재여패초(Echinochloa crusgalli, BYG)공배양,이야생형PI312777위대조。결과발현,여패초공배양하CT829537-OEPI312777적분류대사관건매기인표체상조,근계급기수배액중적총분산농도증가,억초능력증강；상동처리하 CT829537-RNAiPI312777칙상반,기분류대사관건매기인교야생형식주표체하조,총분산농도강저,억초능력하강。저표명OsMYB (CT829537)통과조절화감수도적분산류물질합성진이영향기억초능력。
Regulation of gene expression is a vital process in the formation of rice allelopathy. In this study, allelopathic rice PI312777 (Oryza sativa L.) was modified by RNA interference (RNAi) and overexpression(OE) technologies to inhibit or en-hance gene expression ofOsMYB(CT829537) in PI312777 respectively. The results showed that up-regulation of phenolic syn-thesis related genes was found inCT829537-OE transgenic PI312777 co-cultured with barnyardgrass (BYG), compared with that in wild type. However, the reverse was true in theCT829537-RNAi transgenic PI312777. Up-regulation of the gene expression in CT829537-OE transgenic PI312777 increased phenolic acids contents in rice root and root exudates, which led to enhance allelo-pathic inhibition on barnyardgrass. In contrast, decreases of phenolic acids contents and weed inhibition were found in CT829537-RNAi transgenic PI312777. These results implied thatCT829537 is responsible for regulating phenolic synthesis in allelopathic rice PI312777 and then enhances allelopathic inhibition on weeds.