CAJ | 학술논문

向光素 PHOT1介导较宽范围蓝光诱导的下胚轴向光弯曲，而向光素 PHOT2仅在强蓝光下起作用。强蓝光下， PHOT1和PHOT2介导拟南芥下胚轴向光弯曲的功能冗余性，限制了人们对PHOT2功能的研究。为此，以拟南芥phot1突变体为材料，避开 PHOT1基因的干扰，通过 EMS 诱变筛选拟南芥下胚轴向光不弯曲突变体，成功克隆到1个基因，命名为P2SA2(phototropin 2 signaling associated 2)，该基因被证明是NPH3的等位基因。P2SA2基因的突变可导致拟南芥缺失强蓝光诱导的下胚轴向光弯曲反应。在p2sa2突变体背景下， P2SA2基因超表达可恢复强蓝光诱导的拟南芥下胚轴向光弯曲。该结果将为强蓝光下PHOT2下游基因的筛选、功能鉴定和揭开PHOT2调节强蓝光诱导的下胚轴弯曲的机制提供理论基础。
향광소 PHOT1개도교관범위람광유도적하배축향광만곡，이향광소 PHOT2부재강람광하기작용。강람광하， PHOT1화PHOT2개도의남개하배축향광만곡적공능용여성，한제료인문대PHOT2공능적연구。위차，이의남개phot1돌변체위재료，피개 PHOT1기인적간우，통과 EMS 유변사선의남개하배축향광불만곡돌변체，성공극륭도1개기인，명명위P2SA2(phototropin 2 signaling associated 2)，해기인피증명시NPH3적등위기인。P2SA2기인적돌변가도치의남개결실강람광유도적하배축향광만곡반응。재p2sa2돌변체배경하， P2SA2기인초표체가회복강람광유도적의남개하배축향광만곡。해결과장위강람광하PHOT2하유기인적사선、공능감정화게개PHOT2조절강람광유도적하배축만곡적궤제제공이론기출。
PHOT1 functions at both low- and high-intensity blue light to mediate phototropic responses, but PHOT2 functions only at high-intensity blue light. The functional redundancy of PHOT1 and PHOT2 on high-intensity blue light-induced phototropic curvature of hypocotyls inArabidopsis thaliana, restricts the understanding of the mechanism of PHOT2 signal transduction. Therefore, in order to avoid the interference of PHOT1,Arabidopsisphot1 mutant was selected as material for screening high blue light insensitive mutants by the EMS mutation, and we successfully screened and cloned the gene P2SA2 (phototropin 2 signaling associated 2). The geneP2SA2turned out to be the allelic ofNPH3(Nonphototropic hypocotyl 3). The mutation of geneP2SA2 could result in thatArabidopsis thaliana lost the phototropism to unilateral high intensity blue light. Transgenic plants ofp2sa2 35S::P2SA2restored hypocotyl phototropism to high intensity blue light. These findings will open new perspectives aboutthe screening and functional identification of PHOT2 downstream genes in response to high blue light, and provide the theoretical basis to uncover hypocotyl bending mechanism regulated by PHOT2.