重组鸡痘病毒rFPVHg-Hp理化特性检测
중조계두병독rFPVHg-Hp이화특성검측
Physical and Chemical Factors Detection of the Recombinant Fowl-pox Virus rFPVHg-Hp
  • 万方数据
    生物技术通讯 생물기술통신
    LETTERS IN BIOTECHNOLOGY
    2015年 2期 223-226 ,共4页

    刘继%李昌%朱羿龙%朱光泽%张艳芳%杜寿文%谭鹏%李一权%郭焱%李金泽%邢彬%金宁一

    류계%리창%주예룡%주광택%장염방%두수문%담붕%리일권%곽염%리금택%형빈%금저일

    重组鸡痘病毒%理化因子%病毒滴度 重組鷄痘病毒%理化因子%病毒滴度
    중조계두병독%이화인자%병독적도
    recombinant fowlpox virus%physical and chemical factors%TCID50
    目的：检测各理化因素对重组鸡痘病毒rFPVHg-Hp的影响，为临床前研究奠定基础。方法：将重组鸡痘病毒rFPVHg-Hp经热、酸、碱、乙醚、氯仿、胰蛋白酶、超声破碎、紫外线照射处理后，接种于CEF细胞，通过测定TCID50观察重组鸡痘病毒rFPVHg-Hp毒力活性的变化，由此分析这些理化因素对重组鸡痘病毒rFPVHg-Hp的影响。结果与结论：重组鸡痘病毒rFPVHg-Hp不耐高温，在55℃作用45 min或60℃作用15 min即可完全灭活；但是其对酸和乙醚具有一定的耐受性，在pH值为3~9的范围内，pH值的变化对病毒滴度无明显影响；同时紫外线照射无法使得培养基中的病毒粒子完全灭活；该病毒对氯仿、胰蛋白酶较为敏感，二者均可使病毒滴度下降；在适应的条件下，重组鸡痘病毒rFPVHg-Hp对超声波具有一定的耐受性。
    목적：검측각이화인소대중조계두병독rFPVHg-Hp적영향，위림상전연구전정기출。방법：장중조계두병독rFPVHg-Hp경열、산、감、을미、록방、이단백매、초성파쇄、자외선조사처리후，접충우CEF세포，통과측정TCID50관찰중조계두병독rFPVHg-Hp독력활성적변화，유차분석저사이화인소대중조계두병독rFPVHg-Hp적영향。결과여결론：중조계두병독rFPVHg-Hp불내고온，재55℃작용45 min혹60℃작용15 min즉가완전멸활；단시기대산화을미구유일정적내수성，재pH치위3~9적범위내，pH치적변화대병독적도무명현영향；동시자외선조사무법사득배양기중적병독입자완전멸활；해병독대록방、이단백매교위민감，이자균가사병독적도하강；재괄응적조건하，중조계두병독rFPVHg-Hp대초성파구유일정적내수성。
    Objective: To detect the influence of rFPVHg-Hp which was disposed by physical and chemical factors and provide a foundation for the following clinical study. Methods: The CEF cells were infected by the rFPVHg-Hp which was disposed by heat, acid-base, ether, chloroform, trypsin, ultrasonic waves and ultraviolet rays, and then inspected the changes in virus titer to analyze the influence of rFPVHg-Hp which was disposed by these physical and chemical factors. Results & Conclusion: The results showed that rFPVHg-Hp was sensitive to heat. It was com?pletely inactivated by 55℃, 45 min or 60℃, 15 min. rFPVHg-Hp was tolerant of acid-base and ether, and the titer of rFPVHg-Hp was not significantly affected when the pH was changed in a range of 3~9. The virion in the medium was not completely inactivated by ultraviolet rays, and the titer of rFPVHg-Hp was reduced by chloroform and tryp?sin. rFPVHg-Hp was tolerant of ultrasonic waves in the right conditions.
    원문보기 원문다운로드 사이트로이동
저자의 최근 논문