中国比较医学杂志
中國比較醫學雜誌
중국비교의학잡지
CHINESE JOURNAL OF COMPARATIVE MEDICINE
2015年
4期
48-57
,共10页
陆妍%王亚南%刘慧%马卫国%张继胜%谭玲%李慧慧%林子超%沈志明%王洁%孟凤仙
陸妍%王亞南%劉慧%馬衛國%張繼勝%譚玲%李慧慧%林子超%瀋誌明%王潔%孟鳳仙
륙연%왕아남%류혜%마위국%장계성%담령%리혜혜%림자초%침지명%왕길%맹봉선
中药藤莓汤%CIA模型%AI积分%病理改变%免疫炎性机制
中藥籐莓湯%CIA模型%AI積分%病理改變%免疫炎性機製
중약등매탕%CIA모형%AI적분%병리개변%면역염성궤제
Chinese herbal compound,Tengmei decoction%Rat models%Arthritis,collagen II-induced%Arthritis index,AI%Pathological changes%Immune-inflammatory mechanism
目的：探讨中药藤莓汤对Ⅱ型胶原诱导性关节炎（ collagen－induced arthritis，CIA）大鼠关节滑膜炎症指数、四肢肿胀程度、关节组织炎性浸润的改善情况，以及免疫炎性相关因子影响。方法建立CIA模型，将造模成功的SD大鼠随机分为模型组、阳性药组、中药大、小剂量组，设正常对照组，每组6只，干预治疗12周。每周使用游标卡尺测量四肢踝腕部前后径、左右径，爪掌足垫厚度，以及双后肢的跖关节肿胀最高点宽度，评价其关节积分和四肢肿胀程度。结果①与正常组比较，模型组大鼠关节炎总AI评分及双后肢AI评分显著增加（ P＜0．05或P＜0．01）；左前肢AI评分在10－12周时升高（P＜0．05）；左后肢前后径、左右径、足垫厚度及左后肢跖骨最高点测量值在1－12 w不同时间段增加（P＜0．05或P＜0．01）。与模型组比较，中药大、小剂量组总AI评分及左后肢关节AI评分在6 w后降低（P＜0．05）；阳性药组10－12 w时左前肢AI评分下降（P＜0．05）。②与正常组比较，模型组IL－6、TNF－αmRNA转录及蛋白表达水平显著上调（ P＜0．01）。与模型组比较，阳性药组、中药大、小剂量组IL－6、TNF－αmRNA转录及蛋白表达水平显著下调（ P＜0．01）。③光镜检测结果显示：中药小剂量组明显改善了CIA模型大鼠关节滑膜增生、炎性细胞浸润情况。结论中药藤莓汤改善CIA模型大鼠关节病理损伤的分子机制可能与其抑制滑膜组织免疫炎性因子高表达相关。
目的：探討中藥籐莓湯對Ⅱ型膠原誘導性關節炎（ collagen－induced arthritis，CIA）大鼠關節滑膜炎癥指數、四肢腫脹程度、關節組織炎性浸潤的改善情況，以及免疫炎性相關因子影響。方法建立CIA模型，將造模成功的SD大鼠隨機分為模型組、暘性藥組、中藥大、小劑量組，設正常對照組，每組6隻，榦預治療12週。每週使用遊標卡呎測量四肢踝腕部前後徑、左右徑，爪掌足墊厚度，以及雙後肢的蹠關節腫脹最高點寬度，評價其關節積分和四肢腫脹程度。結果①與正常組比較，模型組大鼠關節炎總AI評分及雙後肢AI評分顯著增加（ P＜0．05或P＜0．01）；左前肢AI評分在10－12週時升高（P＜0．05）；左後肢前後徑、左右徑、足墊厚度及左後肢蹠骨最高點測量值在1－12 w不同時間段增加（P＜0．05或P＜0．01）。與模型組比較，中藥大、小劑量組總AI評分及左後肢關節AI評分在6 w後降低（P＜0．05）；暘性藥組10－12 w時左前肢AI評分下降（P＜0．05）。②與正常組比較，模型組IL－6、TNF－αmRNA轉錄及蛋白錶達水平顯著上調（ P＜0．01）。與模型組比較，暘性藥組、中藥大、小劑量組IL－6、TNF－αmRNA轉錄及蛋白錶達水平顯著下調（ P＜0．01）。③光鏡檢測結果顯示：中藥小劑量組明顯改善瞭CIA模型大鼠關節滑膜增生、炎性細胞浸潤情況。結論中藥籐莓湯改善CIA模型大鼠關節病理損傷的分子機製可能與其抑製滑膜組織免疫炎性因子高錶達相關。
목적：탐토중약등매탕대Ⅱ형효원유도성관절염（ collagen－induced arthritis，CIA）대서관절활막염증지수、사지종창정도、관절조직염성침윤적개선정황，이급면역염성상관인자영향。방법건립CIA모형，장조모성공적SD대서수궤분위모형조、양성약조、중약대、소제량조，설정상대조조，매조6지，간예치료12주。매주사용유표잡척측량사지과완부전후경、좌우경，조장족점후도，이급쌍후지적척관절종창최고점관도，평개기관절적분화사지종창정도。결과①여정상조비교，모형조대서관절염총AI평분급쌍후지AI평분현저증가（ P＜0．05혹P＜0．01）；좌전지AI평분재10－12주시승고（P＜0．05）；좌후지전후경、좌우경、족점후도급좌후지척골최고점측량치재1－12 w불동시간단증가（P＜0．05혹P＜0．01）。여모형조비교，중약대、소제량조총AI평분급좌후지관절AI평분재6 w후강저（P＜0．05）；양성약조10－12 w시좌전지AI평분하강（P＜0．05）。②여정상조비교，모형조IL－6、TNF－αmRNA전록급단백표체수평현저상조（ P＜0．01）。여모형조비교，양성약조、중약대、소제량조IL－6、TNF－αmRNA전록급단백표체수평현저하조（ P＜0．01）。③광경검측결과현시：중약소제량조명현개선료CIA모형대서관절활막증생、염성세포침윤정황。결론중약등매탕개선CIA모형대서관절병리손상적분자궤제가능여기억제활막조직면역염성인자고표체상관。
Objective To explore the effects of Chinese herbal compound, Tengmei decoction, on type II collagen-induced arthritis ( CIA) in rats, and to examine the changes of arthritis index ( AI) , limb swelling, joint tissue inflammatory infiltration, and the effects on immune-inflammatory factors.Methods Sprague-Dawley rat models of arthritis were successfully established by intradermal injection of type II collagen and Freund’ s complete adjuvant.The model rats were randomly divided into model group, positive drug group, and high-and low-dose Chinese medicine groups, 6 rats in each group.The intervention and treatment period was 12 weeks.To measure weekly the anteroposterior and transverse diameters of the rear ankles and wrists, the transverse diameter of the claw foot palm pad, the thickness and the highest point width of hind limb plantar joint swelling, and to evaluate the integrated scores of joints and limbs swelling using a vernier caliper.Results ①Compared with the normal group, the total arthritis scores and hind limbs AI scores of the model group were significantly increased ( P <0.05 or P <0.01 ) .The left forelimb AI scores were significantly increased during 10 -12 weeks ( P <0.05 ) .The anteroposterior and transverse diameters of the left hind limb, the thickness of the highest point measurement of the left hind foot pad metatarsal were significantly increased ( P<0.05 or P<0.01) in different time periods between 1-12 weeks.Compared with the model group, the total scores and the left hind limb joints AI scores of the high-and low-dose drug groups were decreased after 6 weeks (P<0.05).②Compared with the normal control group, levels of mRNA transcription and protein expression of IL-6 and TNF-αwere significantly up-regulated ( P<0.01 ) in the model group.Compared with the model group, the levels of mRNA transcription and the expression of IL-6 and TNF-αproteins were significantly down-regulated in the positive group and Chinese medicine groups ( P <0.01 ) .③ Histological examination showed that the low-dose TCM significantly improved the CIA synovial hyperplasia and inflammatory cell infiltration.Conclusions The molecular mechanism of Chinese herbal compound Tengmei decotion in improving joint pathological injury of CIA rat models may be related to its inhibitory effect on the high expression of immune-inflammatory factors in the synovial tissue of CIA rats.
