CAJ | 학술논문

目的：观察加味二陈汤对ApoE－／－小鼠血脂的影响。方法44只ApoE－／－小鼠以高脂饲喂养4周构建AS小鼠模型，分别用辛伐他汀、加味二陈汤对小鼠灌胃给药，8周后处死小鼠，酶法检测血清血脂；每周称取并记录小鼠体重；油红O染色观察小鼠主动脉管腔内粥样斑块；HE染色观察小鼠主动脉管腔内粥样斑块沉积及肝细胞形态。结果加味二陈汤可显著降低小鼠血清中TCHOL、LDL－C水平（ P＜0．01）；各组小鼠体重均有所增加，但模型组小鼠体重略高于空白对照组和加味二陈汤组；油红O染色结果显示加味二陈汤可显著减少ApoE－／－小鼠主动脉管腔内粥样斑块的面积；小鼠主动脉HE染色示加味二陈汤组小鼠主动脉内有脂质沉积斑块较模型组明显减少；肝细胞切片HE染色发现加味二陈汤组小鼠肝细胞的病变程度较轻，肝小叶结构较清晰。结论加味二陈汤能有效降低ApoE－／－小鼠血清中有关脂质的含量，调节小鼠脂质代谢，缓解动脉粥样硬化进程。
목적：관찰가미이진탕대ApoE－／－소서혈지적영향。방법44지ApoE－／－소서이고지사위양4주구건AS소서모형，분별용신벌타정、가미이진탕대소서관위급약，8주후처사소서，매법검측혈청혈지；매주칭취병기록소서체중；유홍O염색관찰소서주동맥관강내죽양반괴；HE염색관찰소서주동맥관강내죽양반괴침적급간세포형태。결과가미이진탕가현저강저소서혈청중TCHOL、LDL－C수평（ P＜0．01）；각조소서체중균유소증가，단모형조소서체중략고우공백대조조화가미이진탕조；유홍O염색결과현시가미이진탕가현저감소ApoE－／－소서주동맥관강내죽양반괴적면적；소서주동맥HE염색시가미이진탕조소서주동맥내유지질침적반괴교모형조명현감소；간세포절편HE염색발현가미이진탕조소서간세포적병변정도교경，간소협결구교청석。결론가미이진탕능유효강저ApoE－／－소서혈청중유관지질적함량，조절소서지질대사，완해동맥죽양경화진정。
Objective To explore the effects of the Chinese medicine, modified Erchen decoction, on the serum lipid spectrum of ApoE-/-mice, and to explore its possible anti-atherosclerotic mechanism.Methods Forty-four male 7-8-week old ApoE-/-mice were used in this experiment.ApoE-/-mouse models of atherosclerosis were generated by high-cholesterol diet for 4 weeks.And then, they were given simvastatin or modified Erchen decoction by gavage.The body weight of mice was recorded every week, The mice were sacrificed after treated with the drugs for 8 weeks continuously, and the plasma lipid was determined by enzymatic method.The aortic valves and arches were stained with oil red O to depict atherosclerotic plaques and liver structural changes of the mice were examined by pathology.Results Modified Erchen decoction lowered plasma lipid ( including TCHOL and LDL-C ) significantly ( P<0.01 ) .The body weight was increased in the mice of all groups, but it was more pronounced in the mice of model group than in the blank and modified Erchen decoction groups.The serum CHOL and LDL-C levels were significantly lowered in the modified Erchen decoction group (P<0.01).The area of atherosclerotic plaques in the aortic wall was significantly reduced in the mice of modified Erchen decoction group as shown by oil red O staining.The pathological changes of hepatocytes were less severe and the structure of hepatic lobules was better preserved in the mice of modified Erchen decoction group.Conclusions The Chinese medicin modified Erchen decoction can effectively reduce serum lipids, regulate lipid metabolism, and ameliorate the process of atherosclerosis in ApoE-/-mice.