CAJ | 학술논문

目的：研究长期高脂饮食对长爪沙鼠生化及主要脏器组织病理学的影响。方法48只长爪沙鼠随机分为模型组和正常组，每组24只，模型组予高脂饮食喂养，正常组予基础饲料喂养，两组分别于4周、8周及16周时各处理沙鼠8只，比较两组体重、血糖（ Glu）、甘油三酯（ TG）、胆固醇（ CHOL）、高密度脂蛋白胆固醇（ HDL－C）、低密度脂蛋白胆固醇（LDL－C）、尿酸（UA）、肌酐（CREA）、尿素氮（BUN）、丙氨酸氨基转移酶（ALT）、天冬酸氨基转移酶（ AST）、总蛋白（ TP）、白蛋白（ ALB）、总胆红素（ TBil）、淀粉酶（ AMS）水平及主要脏器组织病理学的变化。结果和正常组比较，模型组沙鼠血脂明显升高，肝功能受损，血尿酸升高，16周时血糖明显降低，AMS升高，肾功能则无明显变化。肝组织逐渐出现脂肪变、炎症、肝纤维化及肝硬化，并伴有脾大，肺组织和心肌在后期均出现脂肪变性，损伤明显，胰岛增大伴内分泌细胞增多，小肠和肾脏无明显变化。结论高脂饮食喂养的沙鼠可复制良好的高脂血症、非酒精性脂肪性肝炎肝硬化模型，并且有可能是高脂血症相关的高尿酸血症、肺损伤及心肌损伤等疾病的良好模型。
목적：연구장기고지음식대장조사서생화급주요장기조직병이학적영향。방법48지장조사서수궤분위모형조화정상조，매조24지，모형조여고지음식위양，정상조여기출사료위양，량조분별우4주、8주급16주시각처리사서8지，비교량조체중、혈당（ Glu）、감유삼지（ TG）、담고순（ CHOL）、고밀도지단백담고순（ HDL－C）、저밀도지단백담고순（LDL－C）、뇨산（UA）、기항（CREA）、뇨소담（BUN）、병안산안기전이매（ALT）、천동산안기전이매（ AST）、총단백（ TP）、백단백（ ALB）、총담홍소（ TBil）、정분매（ AMS）수평급주요장기조직병이학적변화。결과화정상조비교，모형조사서혈지명현승고，간공능수손，혈뇨산승고，16주시혈당명현강저，AMS승고，신공능칙무명현변화。간조직축점출현지방변、염증、간섬유화급간경화，병반유비대，폐조직화심기재후기균출현지방변성，손상명현，이도증대반내분비세포증다，소장화신장무명현변화。결론고지음식위양적사서가복제량호적고지혈증、비주정성지방성간염간경화모형，병차유가능시고지혈증상관적고뇨산혈증、폐손상급심기손상등질병적량호모형。
Objective To explore the effect of high fat diet on serum biochemical parameters and histopathology of main organs in Mongolian gerbils.Methods Forty-eight healthy adult male Mongolian gerbils were randomly and equally divided into model and normal groups.The gerbils in the model group were fed with high fat diet while the normal group with standard diet.Eight gerbils in each group were killed at the end of 4th,8th and 16th week,respectively,and the body weight, serum levels of Glu, TG, CHOL, HDL-C, LDL-C, UA, CREA, BUN, TBil, TP, ALB, ALT, AST and AMS were determined.The histopathological changes of main organs were observed.Results Compared with the normal group,the blood lipid of the model gerbils was significantly increased, the liver function was impaired, the blood uric acid level was higher, and the blood glucose was decreased at the end of 16th week.The AMS was increased at the end of 16th week,but the renal function showed no significant changes.The liver tissue of the model group gradually showed steatosis, inflammation, fibrosis and cirrhosis, accompanied by splenomegalia. The lung tissue and myocardium showed fatty degeneration and obvious damages in the later period,the pancreatic islets were enlarged and the amount of endocrine cells was increased,and the small intestine and kidney didn’ t show any distinct changes.Conclusions A gerbil models of hyperlipidemia and nonalcoholic steatohepatitis and cirrhosis can be well established by high fat diet feeding,and may serve as good models for research of hyperlipidemia-related hyperuricemia, and lung and myocardial damages.