CAJ | 학술논문

目的：探讨湿化学酶法检测华氏巨球蛋白血症患者肌酐假性增高的原因及解决方法。方法以2010～2012年中国人民解放军白求恩国际和平医院收治的5例华氏巨球蛋白血症患者为研究对象，采用离心超滤管将采集的患者血清中大分子蛋白滤去，分别用湿化学酶法、苦味酸法、干化学酶法检测超滤前后患者血清肌酐水平并进行比较分析。结果超滤前其中2例华氏巨球蛋白血症患者用湿化学酶法测得血清肌酐水平与苦味酸法、干化学酶法比较有明显差异，而5例患者后2种方法测得的肌酐水平差异不明显。而以离心超滤管滤去大分子蛋白后，3种方法测得的血清肌酐水平无明显差异。结论采用湿化学酶法检测华氏巨球蛋白血症患者血清肌酐时，应去除大分子蛋白以防止所测血清肌酐水平异常增高，或用干化学酶法或苦味酸法测定以确定检测的准确性，为临床提供真实可靠的检验结果。
목적：탐토습화학매법검측화씨거구단백혈증환자기항가성증고적원인급해결방법。방법이2010～2012년중국인민해방군백구은국제화평의원수치적5례화씨거구단백혈증환자위연구대상，채용리심초려관장채집적환자혈청중대분자단백려거，분별용습화학매법、고미산법、간화학매법검측초려전후환자혈청기항수평병진행비교분석。결과초려전기중2례화씨거구단백혈증환자용습화학매법측득혈청기항수평여고미산법、간화학매법비교유명현차이，이5례환자후2충방법측득적기항수평차이불명현。이이리심초려관려거대분자단백후，3충방법측득적혈청기항수평무명현차이。결론채용습화학매법검측화씨거구단백혈증환자혈청기항시，응거제대분자단백이방지소측혈청기항수평이상증고，혹용간화학매법혹고미산법측정이학정검측적준학성，위림상제공진실가고적검험결과。
Objective To explore causes and solutions of false estimates of elevated serum creatinine in patients with Walden‐strom′s macroglobulinemia throug wet chemical enzymatic method .Methods 5 cases of patients hospitalized in the Bethune Inter‐national Peace Hospital were enrolled as subjects from 2010 to 2012 .The large molecular proteins were removal from serum sam‐ples collected from patients with Waldenstrom′s macroglobulinemia by using centrifugal ultrafiltration tube .The serum creatinine levels were detected through using the wet chemical enzymatic method ,wet chemical picric acid method and dry chemical enzymatic method before and after ultrafiltration ,and data were compared .Results Before ultrafiltration ,the levels of serum creatinine of 2 cases of patients with Waldenstrom′s macroglobulinemia detected by using wet chemical enzymatic method differed with those de‐tected by using wet chemical picric acid method and dry chemical enzymatic method .While there were no obvious differences be‐tween levels of serum creatinine detected by wet chemical picric acid method and dry chemical enzymatic method .While ,after ultra‐filtration ,no obvious differences were founded in levels of serum creatinine detected by the thress methods .Conclusion The large molecular proteins should be eliminated when using the wet chemical enzymatic method in the detection of serum creatinine levels , in order to avoid abnormal increase .And the wet chemical picric acid method and dry chemical enzymatic method could also be uti‐lized to determine the accuracy ,and provide reliable determination results .