CAJ | 학술논문

目的：探讨长春西汀对小鼠肾缺血再灌注（IR ）损伤的保护作用及其作用机制。方法将48只健康小鼠分成假手术组、IR组、长春西汀低剂量干预组和长春西汀高剂量干预组，采用双侧肾蒂夹闭45 min的方法建立小鼠肾IR损伤模型，HE染色后观察肾脏组织形态学变化，测定再灌注24 h后各组血肌酐（SCr）、尿素氮（BUN）、肿瘤坏死因子（TNF‐α）和白细胞介素‐1β（IL‐1β）和IL‐6水平，观察肾脏组织中超氧化物歧化酶（SOD）活性和丙二醛（MDA）水平。结果与假手术组相比，IR组出现肾小管管腔扩张，刷状缘消失，管腔内有管型等病理改变，血清中SCr、BUN、TNF‐α、IL‐1β和IL‐6水平明显升高，差异均有统计学意义（P＜0．05），肾组织SOD活性降低，差异有统计学意义（P＜0．05），MDA水平升高，差异也有统计学意义（P＜0．05）；与IR组相比，长春西汀10 mg／kg和20 mg／kg干预可呈剂量依赖性改善肾组织病理损伤，SCr、BUN、TNF‐α、IL‐1β、IL‐6和MDA水平减少，使肾组织SOD活性升高，差异有统计学意义（P＜0．05）。结论长春西汀可通过抑制炎症和氧化应激来减轻肾IR损伤。
목적：탐토장춘서정대소서신결혈재관주（IR ）손상적보호작용급기작용궤제。방법장48지건강소서분성가수술조、IR조、장춘서정저제량간예조화장춘서정고제량간예조，채용쌍측신체협폐45 min적방법건립소서신IR손상모형，HE염색후관찰신장조직형태학변화，측정재관주24 h후각조혈기항（SCr）、뇨소담（BUN）、종류배사인자（TNF‐α）화백세포개소‐1β（IL‐1β）화IL‐6수평，관찰신장조직중초양화물기화매（SOD）활성화병이철（MDA）수평。결과여가수술조상비，IR조출현신소관관강확장，쇄상연소실，관강내유관형등병리개변，혈청중SCr、BUN、TNF‐α、IL‐1β화IL‐6수평명현승고，차이균유통계학의의（P＜0．05），신조직SOD활성강저，차이유통계학의의（P＜0．05），MDA수평승고，차이야유통계학의의（P＜0．05）；여IR조상비，장춘서정10 mg／kg화20 mg／kg간예가정제량의뢰성개선신조직병리손상，SCr、BUN、TNF‐α、IL‐1β、IL‐6화MDA수평감소，사신조직SOD활성승고，차이유통계학의의（P＜0．05）。결론장춘서정가통과억제염증화양화응격래감경신IR손상。
Objective To investigate the protective effect and mechanism of vinpocetine in renal ischemia‐reperfusion injury .Methods Forty‐eight healthy male mice were randomly divided into the sham operation group ,is‐chemia‐reperfusion (IR) group ,low dose and high dose(10 mg/kg and 20 mg/kg) vinpocetine pretreatment groups . The renal ischemia‐reperfusion injury models were established by clamping bilateral renal pedicle for 45 min .The re‐nal morphology changes were observed by the HE staining .The Scr ,BUN ,TNF‐α,IL‐1βand IL‐6 levels at 24 h after reperfusion were detected .The superoxide dismutase (SOD) activity and malondialdehyde (MDA) contentrations were determined .Results Compared with the sham operation group ,the pathological changes in the IR group were tubular dilatation ,loss of brush border and casts in lumen .The levels of Scr ,BUN ,TNF‐α,IL‐1βand IL‐6 in the IR group were significantly increased(P<0 .05) .The SOD activity of kidney tissue was significantly decreased and the MDA contentration was increased ,the differences were statistical significant(P<0 .05);compared with the IR group , vinpocetine10 mg/kg and 20 mg/kg could improve the pathological injury of renal tissue in a dose‐dependent manner , the levels of Scr ,BUN ,TNF‐α,IL‐1β,IL‐6 and MDA were decreased and the SOD activity was increased ,the differ‐ences were statistically significant(P<0 .05) .Conclusion Vinpocetine can ameliorate the renal ischemia‐reperfusion injury in mice by inhibiting inflammation and oxidative stress .