徐州医学院学报
徐州醫學院學報
서주의학원학보
ACTA ACADEMIAE MEDICINAE XUZHOU
2015年
3期
146-151
,共6页
戴利%庞庆丰%张鹏%秦迎松
戴利%龐慶豐%張鵬%秦迎鬆
대리%방경봉%장붕%진영송
急性肝损伤%百草枯%亚甲蓝
急性肝損傷%百草枯%亞甲藍
급성간손상%백초고%아갑람
acute liver injury%paraquat%methylene blue
目的：探讨亚甲蓝对大鼠百草枯中毒诱导的急性肝损伤的保护作用并对保护机制进行研究。方法24只清洁级雄性成年 SD 大鼠随机分为正常组、亚甲蓝（MB）组、百草枯（PQ）组和亚甲蓝治疗（PQ ＋MB）组，每组6只。腹腔内注射 PQ 35 mg／kg 建立大鼠中毒模型，PQ ＋MB 组大鼠于造模2 h 后腹腔内注射 MB 2 mg／kg，MB 组大鼠腹腔内注射 MB 2 mg／kg，正常组大鼠腹腔内注射等量无菌生理盐水。治疗后48 h 收集大鼠血清检测谷丙转氨酶（alanine transaminase， ALT）、谷草转氨酶（aspartate aminotransferase， AST）和碱性磷酸酶（alkaline phospha－tase， ALP），肝组织用于病理学分析、髓过氧化物酶（myeloperoxidase， MPO）活性、丙二醛（malondialdehyde， MDA）含量、超氧化物歧化酶（superoxide dismutase，SOD）活性、三磷酸腺苷（adenosine triphosphate，ATP）含量和血红素加氧酶－1（heme oxygenase －1，HO －1）免疫组化检测。另取40只大鼠，按照上述分组方法评估72 h 内死亡时间和死亡率。结果与正常组和 MB 组比较，PQ 组 ALT 和 ALP 水平增高， MPO 活性增强，MDA 含量增加， SOD 活性降低，ATP 含量降低和 HO －1表达增加（P ＜0．05）；MB 治疗后能够改变上述生化指标变化，减轻肝病理形态学变化，与 PQ 组相比，PQ ＋MB 组 HO －1表达量增高，同时大鼠生存时间延长，差异均具有统计学意义（P ＜0．05）。结论MB 能够减轻氧化损伤和抑制炎症反应，对 PQ 诱导的大鼠急性肝损伤发挥保护作用。
目的：探討亞甲藍對大鼠百草枯中毒誘導的急性肝損傷的保護作用併對保護機製進行研究。方法24隻清潔級雄性成年 SD 大鼠隨機分為正常組、亞甲藍（MB）組、百草枯（PQ）組和亞甲藍治療（PQ ＋MB）組，每組6隻。腹腔內註射 PQ 35 mg／kg 建立大鼠中毒模型，PQ ＋MB 組大鼠于造模2 h 後腹腔內註射 MB 2 mg／kg，MB 組大鼠腹腔內註射 MB 2 mg／kg，正常組大鼠腹腔內註射等量無菌生理鹽水。治療後48 h 收集大鼠血清檢測穀丙轉氨酶（alanine transaminase， ALT）、穀草轉氨酶（aspartate aminotransferase， AST）和堿性燐痠酶（alkaline phospha－tase， ALP），肝組織用于病理學分析、髓過氧化物酶（myeloperoxidase， MPO）活性、丙二醛（malondialdehyde， MDA）含量、超氧化物歧化酶（superoxide dismutase，SOD）活性、三燐痠腺苷（adenosine triphosphate，ATP）含量和血紅素加氧酶－1（heme oxygenase －1，HO －1）免疫組化檢測。另取40隻大鼠，按照上述分組方法評估72 h 內死亡時間和死亡率。結果與正常組和 MB 組比較，PQ 組 ALT 和 ALP 水平增高， MPO 活性增彊，MDA 含量增加， SOD 活性降低，ATP 含量降低和 HO －1錶達增加（P ＜0．05）；MB 治療後能夠改變上述生化指標變化，減輕肝病理形態學變化，與 PQ 組相比，PQ ＋MB 組 HO －1錶達量增高，同時大鼠生存時間延長，差異均具有統計學意義（P ＜0．05）。結論MB 能夠減輕氧化損傷和抑製炎癥反應，對 PQ 誘導的大鼠急性肝損傷髮揮保護作用。
목적：탐토아갑람대대서백초고중독유도적급성간손상적보호작용병대보호궤제진행연구。방법24지청길급웅성성년 SD 대서수궤분위정상조、아갑람（MB）조、백초고（PQ）조화아갑람치료（PQ ＋MB）조，매조6지。복강내주사 PQ 35 mg／kg 건립대서중독모형，PQ ＋MB 조대서우조모2 h 후복강내주사 MB 2 mg／kg，MB 조대서복강내주사 MB 2 mg／kg，정상조대서복강내주사등량무균생리염수。치료후48 h 수집대서혈청검측곡병전안매（alanine transaminase， ALT）、곡초전안매（aspartate aminotransferase， AST）화감성린산매（alkaline phospha－tase， ALP），간조직용우병이학분석、수과양화물매（myeloperoxidase， MPO）활성、병이철（malondialdehyde， MDA）함량、초양화물기화매（superoxide dismutase，SOD）활성、삼린산선감（adenosine triphosphate，ATP）함량화혈홍소가양매－1（heme oxygenase －1，HO －1）면역조화검측。령취40지대서，안조상술분조방법평고72 h 내사망시간화사망솔。결과여정상조화 MB 조비교，PQ 조 ALT 화 ALP 수평증고， MPO 활성증강，MDA 함량증가， SOD 활성강저，ATP 함량강저화 HO －1표체증가（P ＜0．05）；MB 치료후능구개변상술생화지표변화，감경간병리형태학변화，여 PQ 조상비，PQ ＋MB 조 HO －1표체량증고，동시대서생존시간연장，차이균구유통계학의의（P ＜0．05）。결론MB 능구감경양화손상화억제염증반응，대 PQ 유도적대서급성간손상발휘보호작용。
Objective To discuss the protective effects of methylene blue (MB) on paraquat (PQ) -induced acute liver injury and potential mechanisms.Methods A total of 24 male Sprague -Dawley rats were divided into four groups according to their different treatments (n =6): a normal group intraperitioneally injected with sterile saline, a methylene blue (MB) group administrated with 2 mg/kg MB i.p.alone, a paraquat (PQ) model group exposed to 35 mg/kg PQ i. p.alone, and a treatment group receiving 2 mg/kg MB 2 hour after intraperitioneal injection of 35 mg/lg PQ.Then ser-um samples were collected from the rats two days after treatment to examine their levels of alanine transaminase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP).The liver tissues were taken to determine the activi-ties of myeloperoxidase (MPO) and superoxide dismutase (SOD) as well as the amounts of malondialdehyde (MDA) and adenosine triphosphate (ATP), in addition to pathological analysis.The expression of hemeoxygenase -1 (HO -1) was measured by immunohistochemistry.Furthermore, another forty rats were used to examine their survival times and rates after exposure to the same agents mentioned above.Results Compared with the normal or MB group, the PQ group produced significant increases in the quantities of MDA and HO -1 as well as the activities of MPO, ALT and ALP, in comparison with reduced levels of SOD and ATP (P <0.05), which were then reversed after MB treatment.Moreover, MB could alleviate PQ -induced pathological damages.Meanwhile, the combination of PQ and MB resulted in higher a-mounts of HO -1 and longer survival times than administration with PQ alone (P <0.05).Conclusion MB can protect rats from PQ -induced acute liver injury, which may be associated with its abilities to relieve oxidative damages and in-hibit inflammation.
