国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2015年
8期
1115-1116,1119
,共3页
甲胎蛋白%DNA倍体%原发性肝癌%预后
甲胎蛋白%DNA倍體%原髮性肝癌%預後
갑태단백%DNA배체%원발성간암%예후
α-fetoprotein%DNA ploidy%primary hepatic carcinoma%prognosis
目的：研究惠州市博罗地区原发性肝癌（PHC）患者血清甲胎蛋白（AFP）水平和DNA倍体类型与PHC患者5年生存率及生存期的关系，评价其对PHC的预后价值。方法选取80例PHC患者纳入观察组，根据病理分级分为Ⅰ、Ⅱ、Ⅲ、Ⅳ级；另选取80例体检健康者纳入对照组。采集所有受试者的外周静脉血2 mL ，采用电化学发光免析法测定血清AFP水平，流式细胞术测定DNA倍体类型、DNA指数及S期细胞比率。结果不同病理分级PHC患者AFP水平均高于对照组，组间比较差异均有统计学意义（P＜0．05），且随着PHC患者病理分级的增加而升高；患者血清AFP的升高、DNA倍体类型的不同和PHC的病理分级对患者的5年生存率和生存期都有很大的影响。结论惠州市博罗地区人群中，PHC患者血清AFP水平与DNA异倍体阳性率的检测对PHC的早期诊断和预后判断具有重要作用。
目的：研究惠州市博囉地區原髮性肝癌（PHC）患者血清甲胎蛋白（AFP）水平和DNA倍體類型與PHC患者5年生存率及生存期的關繫，評價其對PHC的預後價值。方法選取80例PHC患者納入觀察組，根據病理分級分為Ⅰ、Ⅱ、Ⅲ、Ⅳ級；另選取80例體檢健康者納入對照組。採集所有受試者的外週靜脈血2 mL ，採用電化學髮光免析法測定血清AFP水平，流式細胞術測定DNA倍體類型、DNA指數及S期細胞比率。結果不同病理分級PHC患者AFP水平均高于對照組，組間比較差異均有統計學意義（P＜0．05），且隨著PHC患者病理分級的增加而升高；患者血清AFP的升高、DNA倍體類型的不同和PHC的病理分級對患者的5年生存率和生存期都有很大的影響。結論惠州市博囉地區人群中，PHC患者血清AFP水平與DNA異倍體暘性率的檢測對PHC的早期診斷和預後判斷具有重要作用。
목적：연구혜주시박라지구원발성간암（PHC）환자혈청갑태단백（AFP）수평화DNA배체류형여PHC환자5년생존솔급생존기적관계，평개기대PHC적예후개치。방법선취80례PHC환자납입관찰조，근거병리분급분위Ⅰ、Ⅱ、Ⅲ、Ⅳ급；령선취80례체검건강자납입대조조。채집소유수시자적외주정맥혈2 mL ，채용전화학발광면석법측정혈청AFP수평，류식세포술측정DNA배체류형、DNA지수급S기세포비솔。결과불동병리분급PHC환자AFP수평균고우대조조，조간비교차이균유통계학의의（P＜0．05），차수착PHC환자병리분급적증가이승고；환자혈청AFP적승고、DNA배체류형적불동화PHC적병리분급대환자적5년생존솔화생존기도유흔대적영향。결론혜주시박라지구인군중，PHC환자혈청AFP수평여DNA이배체양성솔적검측대PHC적조기진단화예후판단구유중요작용。
Objective To investigate the relationship between the serum level of AFP ,types of DNA ploidy and 5 year survival rate ,survival time in patients with primary hepatic carcinoma (PHC) in Boluo area in Huizhou City ,and evaluate the prognosis val‐ue of these indexes in PHC .Methods 80 cases with PHC were enrolled in observation group and divided into grade I ,II ,III and IV according to pathological grading .Other 80 cases of healthy individuals ,conducted physical examination ,were enrolled in control group .Peripheral venous blood samples (2 mL) were collected from all subjects .The serum level of AFP and types of DNA ploidy were measured by electrochemiluminescence immunoassay (ECLIA) and flow cytometry ,respectively .Results The serum levels of AFP were significantly higher in patients in different pathological grade groups compared to that in the control group (P<0 .05) .In addition ,accompany increase of pathological grade ,the levels of AFP in patients with PHC were increased .The increased level of AFP ,difference of DNA ploid types and pathological grade of PHC contributed great influences on the 5 year survival rate and sur‐vival time of patients .Conclusion The serum level of AFP and the DNA heteroploid rate may play important roles in the early di‐agnosis and assessing prognosis of patients with PHC in Boluo area in Huizhou City .
