徐州医学院学报
徐州醫學院學報
서주의학원학보
ACTA ACADEMIAE MEDICINAE XUZHOU
2015年
3期
158-161
,共4页
黄传锋%朱一硕%许宁%范月超
黃傳鋒%硃一碩%許寧%範月超
황전봉%주일석%허저%범월초
胶质瘤细胞%SMYD3%增殖%迁移%侵袭
膠質瘤細胞%SMYD3%增殖%遷移%侵襲
효질류세포%SMYD3%증식%천이%침습
glioma cells%SMYD3%proliferation%migration%invasion
目的:探讨 SET -及 MYND -结构域含有蛋白3(SMYD3)siRNA 对胶质瘤细胞迁移、侵袭和增殖的影响。方法将 Control siRNA、SMYD3 siRNA 分别转染脑胶质瘤 U87细胞,Western blot 实验检测 SMYD3蛋白表达情况,CCK -8细胞增殖实验检测沉默 SMYD3对 U87细胞增殖的影响,Transwell chamber 细胞迁移、侵袭实验观察沉默 SMYD3对 U87细胞迁移、侵袭的影响。结果SMYD3 siRNA 组 SMYD3蛋白相对表达量为0.377±0.252,Control siRNA 组为0.843±0.02;CCK -8细胞增殖实验中,24、48、72、96 h SMYD3 siRNA 组细胞的光密度(D)值分别为0.677±0.130、0.920±0.061、1.495±0.01、1.986±0.138,Control siRNA 组分别为0.375±0.041、0.649±0.071、0.944±0.08、1.256±0.130;Transwell chamber 细胞迁移、侵袭实验中,SMYD3 siRNA 组细胞迁移能力下降了36.7%,细胞的侵袭能力下降了约41.9%(P 均<0.05)。结论抑制 SMYD3表达后,胶质瘤 U87细胞的增殖、迁移和侵袭能力下降。
目的:探討 SET -及 MYND -結構域含有蛋白3(SMYD3)siRNA 對膠質瘤細胞遷移、侵襲和增殖的影響。方法將 Control siRNA、SMYD3 siRNA 分彆轉染腦膠質瘤 U87細胞,Western blot 實驗檢測 SMYD3蛋白錶達情況,CCK -8細胞增殖實驗檢測沉默 SMYD3對 U87細胞增殖的影響,Transwell chamber 細胞遷移、侵襲實驗觀察沉默 SMYD3對 U87細胞遷移、侵襲的影響。結果SMYD3 siRNA 組 SMYD3蛋白相對錶達量為0.377±0.252,Control siRNA 組為0.843±0.02;CCK -8細胞增殖實驗中,24、48、72、96 h SMYD3 siRNA 組細胞的光密度(D)值分彆為0.677±0.130、0.920±0.061、1.495±0.01、1.986±0.138,Control siRNA 組分彆為0.375±0.041、0.649±0.071、0.944±0.08、1.256±0.130;Transwell chamber 細胞遷移、侵襲實驗中,SMYD3 siRNA 組細胞遷移能力下降瞭36.7%,細胞的侵襲能力下降瞭約41.9%(P 均<0.05)。結論抑製 SMYD3錶達後,膠質瘤 U87細胞的增殖、遷移和侵襲能力下降。
목적:탐토 SET -급 MYND -결구역함유단백3(SMYD3)siRNA 대효질류세포천이、침습화증식적영향。방법장 Control siRNA、SMYD3 siRNA 분별전염뇌효질류 U87세포,Western blot 실험검측 SMYD3단백표체정황,CCK -8세포증식실험검측침묵 SMYD3대 U87세포증식적영향,Transwell chamber 세포천이、침습실험관찰침묵 SMYD3대 U87세포천이、침습적영향。결과SMYD3 siRNA 조 SMYD3단백상대표체량위0.377±0.252,Control siRNA 조위0.843±0.02;CCK -8세포증식실험중,24、48、72、96 h SMYD3 siRNA 조세포적광밀도(D)치분별위0.677±0.130、0.920±0.061、1.495±0.01、1.986±0.138,Control siRNA 조분별위0.375±0.041、0.649±0.071、0.944±0.08、1.256±0.130;Transwell chamber 세포천이、침습실험중,SMYD3 siRNA 조세포천이능력하강료36.7%,세포적침습능력하강료약41.9%(P 균<0.05)。결론억제 SMYD3표체후,효질류 U87세포적증식、천이화침습능력하강。
Objective To investigate the effects of SET and MYND domain containing 3 (SMYD3) small interfering RNA (siRNA) on the migration, invasion and proliferation of glioma cells.Methods Both control siRNA and SMYD3 siRNA were transfected into U87 glioma cells.Then, the amount of SMYD3 in U87 glioma cells was detected by Western blotting.The proliferation of U87 glioma cells after SMYD3 knockdown was measured by CCK -8 assay.Furthermore, the effects of SMYD3 on the migration and invasion of U87 glioma cells were determined using Transwell chamber and in-vasion assay.Results The relative amount of SMYD3 was 0.377 ±0.252 in the silencing group and 0.843 ±0.02 in the control.According to CCK -8 assay, after 24 h, 48 h, 72 h and 96 h of cultivation, the D values of SMYD3 siRNA/control siRNA were 0.677 ±0.130/0.375 ±0.041, 0.920 ±0.061/0.649 ±0.071, 1.495 ±0.01 /0.944 ±0.08 and 1.986 ±0.138 /1.256 ±0.130, respectively.After SMYD3 silencing, U87 glioma cells presented remarkably decreases in its abilities to migration (36.7%, P <0.05) and invasion (41.9%, P <0.05).Conclusion Knockdown of SMYD3 can suppress the migration, invasion and proliferation of U87 glioma cells.