CAJ | 학술논문

目的：利用多重聚合酶链反应（PCR）技术，建立一种可以同时快速检测肠致病性大肠埃希菌（EPEC ）、肠侵袭性大肠埃希菌（EIEC）、肠出血性大肠埃希菌（EHEC）3种致泻性大肠埃希菌的多重PCR方法。方法根据EPEC的eae基因、EIEC的ipaH基因、EHEC的stx1基因筛选设计引物，建立多重PCR检测体系，并对PCR反应体系和条件进行优化。结果设计的3对PCR引物均能特异地扩增出相应的目的基因，该多重PCR体系能同时检测3种目的菌，特异度强。结论初步建立了一种能够同时快速检测3种致泻性大肠埃希菌的多重PCR检测方法，可用于食品安全及食物中毒事件的快速筛查。
목적：이용다중취합매련반응（PCR）기술，건립일충가이동시쾌속검측장치병성대장애희균（EPEC ）、장침습성대장애희균（EIEC）、장출혈성대장애희균（EHEC）3충치사성대장애희균적다중PCR방법。방법근거EPEC적eae기인、EIEC적ipaH기인、EHEC적stx1기인사선설계인물，건립다중PCR검측체계，병대PCR반응체계화조건진행우화。결과설계적3대PCR인물균능특이지확증출상응적목적기인，해다중PCR체계능동시검측3충목적균，특이도강。결론초보건립료일충능구동시쾌속검측3충치사성대장애희균적다중PCR검측방법，가용우식품안전급식물중독사건적쾌속사사。
Objective To establish a multiplex polymerase chain reaction (PCR) method for rapid detection of three kinds of di‐arrheagenic Escherichia coli(EPEC ,EIEC ,EHEC)simultaneously .Methods The eae gene of EPEC ,ipaH gene of EIEC and stx1 gene of EHEC were selected to design primers ;the reaction system and condition were adjusted to optimize the multiplex PCR sys‐tem .Results The target gene fragments were amplified correctly with these primers .The three target bacteria could be detected at the same time by multiplex PCR .Conclusion A rapid multiplex PCR system were successfully established for detection of three di‐arrheagenic Escherichia coli ,and this system could be suitable for rapid screening in food safety .