CAJ | 학술논문

目的：对分离自同一患者不同血流部位的3株鲍曼不动杆菌进行同源性分析，在基因水平上明确中心静脉导管相关血流感染的实验室诊断，为临床防治提供依据。方法采用西门子Microscan Walkaway 40 Plus微生物鉴定及药敏系统对分离的3株鲍曼不动杆菌进行细菌鉴定及药敏分析，应用肠杆菌科基因组间重复序列聚合酶链反应（ERIC‐PCR）及琼脂糖凝胶电泳进行同源性分析。结果3株鲍曼不动杆菌ERIC‐PCR均扩增出阳性产物，3株细菌均电泳出13条位置相同的条带。结论3株鲍曼不动杆菌属于相同的基因型，ERIC‐PCR可用于鲍曼不动杆菌的同源性分析。
목적：대분리자동일환자불동혈류부위적3주포만불동간균진행동원성분석，재기인수평상명학중심정맥도관상관혈류감염적실험실진단，위림상방치제공의거。방법채용서문자Microscan Walkaway 40 Plus미생물감정급약민계통대분리적3주포만불동간균진행세균감정급약민분석，응용장간균과기인조간중복서렬취합매련반응（ERIC‐PCR）급경지당응효전영진행동원성분석。결과3주포만불동간균ERIC‐PCR균확증출양성산물，3주세균균전영출13조위치상동적조대。결론3주포만불동간균속우상동적기인형，ERIC‐PCR가용우포만불동간균적동원성분석。
Objective To analyze the homology of 3 strains of Acinetobacter baumannii isolated from different bloodstream positions in the same patient for definiting the laboratory diagnosis of central venous catheter‐related bloodstream infection at the gene level to provide the basis for clinical prevention and treatment .Methods 3 strains of Acinetobacter baumannii were identified and the drug sensitivity was performed by using Microscan Walkaway 40Plus microbiological idetification and drug susceptibility system(Sienems ,German) .The homogeneous genotyping was performed by using enterobacteria repetitive intergenic consensus‐polymerase chain reaction (ERIC‐PCR) and Agarose gel electrophoresis .Results The positive products were amplified from 3 strains of Acinetobacter bauman‐nii .13 bands with the same location appeared by electrophoresis .Conclusion 3 strains of Acinetobacter baumannii belong to the same genotype .ERIC‐PCR can be used in the homology analysis of Acinetobacter baumannii .