CAJ | 학술논문

目的：通过对872例强直性脊柱炎（AS）疑似患者 HLA‐B27抗原的检测，分析 HLA‐B27抗原与 AS的相关性及其临床意义。方法选取872例AS疑似患者外周血，采用流式细胞仪检测T淋巴细胞膜上的HLA‐B27／B7抗原，并对患者的表达情况进行分析。结果872例AS疑似患者男女比例为1．8∶1，HLA‐B27抗原总阳性率为27．29％，，男女患者阳性率分别为32．50％和17．95％（ P＜0．05），男女患者B27＋／B7－单抗表达率（％）分别为39．16±42．79和20．96±33．86（ P＜0．05），平均荧光强度分别为5．35 ± 5．44和3．35 ± 3．87（P＜0．05），差别显著。结论用流式细胞技术检测 T淋巴细胞 HLA‐B27的有助于AS的诊断和鉴别诊断。
목적：통과대872례강직성척주염（AS）의사환자 HLA‐B27항원적검측，분석 HLA‐B27항원여 AS적상관성급기림상의의。방법선취872례AS의사환자외주혈，채용류식세포의검측T림파세포막상적HLA‐B27／B7항원，병대환자적표체정황진행분석。결과872례AS의사환자남녀비례위1．8∶1，HLA‐B27항원총양성솔위27．29％，，남녀환자양성솔분별위32．50％화17．95％（ P＜0．05），남녀환자B27＋／B7－단항표체솔（％）분별위39．16±42．79화20．96±33．86（ P＜0．05），평균형광강도분별위5．35 ± 5．44화3．35 ± 3．87（P＜0．05），차별현저。결론용류식세포기술검측 T림파세포 HLA‐B27적유조우AS적진단화감별진단。
Objective To investigate the relationship between suspected ankylosing spondylitis and HLA‐B27 antigen by detec‐ting the positive frequency of HLA‐B27antigen in 872 suspected AS patients ,and evaluate its clinical significance .Methods The positive frequency of HLA‐B27 on the T lymphocyte membrane were detected by flow eytometer in 872 suspected AS patients .Re‐sults Among the 872 suspected AS patients the ratio between male and female was 1 .8∶1 ,the positive rate of antigen HLA‐B27 was 27 .29% ,and the male and female patients′positive rates of HLA‐B27 antigen were 32 .50% and 17 .95% ,respectively (P<0 .05) .The male and female patients′ expression percentage of B27+ /B7- monoclonal antibody were 39 .16 ± 42 .79 and 20 .96 ± 33 .86 ,respectively(P<0 .05) .The male and female patients′mean fluorescence intension of B27+ /B7- monoclonal antibody were 5 .35 ± 5 .44 and 3 .35 ± 3 .87 ,respectively(P<0 .05) .Conclusion The patients with AS are strongly associated with HLA‐B27 an‐tigen .Detection of HLA‐B27 antigen expression intensity in suspected AS patients with FCM is helpful to diagnosis and differential diagnosis of AS .