CAJ | 학술논문

目的：了解H IV感染者脑脊液淋巴细胞亚群分布，探讨脑脊液淋巴细胞亚群在H IV中枢神经系统并发症发生中的意义。方法选取 HIV感染者34例，其中20例无神经系统症状（单纯HIV组），14例伴有神经系统症状（神经系统HIV组），另选取15例健康人（健康对照组）。使用流式细胞术检测脑脊液淋巴细胞亚群，采用免疫比浊法检测IgG水平，并进行比较分析。结果单纯HIV组和神经系统HIV组脑脊液CD8＋ T淋巴细胞百分率高于健康对照组，CD4＋ T淋巴细胞百分率低于健康对照组，HIV感染者IgG水平高于健康对照组，差异均有统计学意义（P＜0．01）；而B淋巴细胞、NK细胞百分率差异均无统计学意义（P＞0．05）；单纯 HIV组和神经系统HIV组间脑脊液各淋巴细胞亚群百分率差异无统计学意义（P＞0．05）。结论 HIV感染者在早期未发生神经系统并发症时就出现脑脊液免疫功能紊乱，淋巴细胞亚群变化趋势与外周血中一致，T 淋巴细胞亚群可能与H IV神经系统症状相关。
목적：료해H IV감염자뇌척액림파세포아군분포，탐토뇌척액림파세포아군재H IV중추신경계통병발증발생중적의의。방법선취 HIV감염자34례，기중20례무신경계통증상（단순HIV조），14례반유신경계통증상（신경계통HIV조），령선취15례건강인（건강대조조）。사용류식세포술검측뇌척액림파세포아군，채용면역비탁법검측IgG수평，병진행비교분석。결과단순HIV조화신경계통HIV조뇌척액CD8＋ T림파세포백분솔고우건강대조조，CD4＋ T림파세포백분솔저우건강대조조，HIV감염자IgG수평고우건강대조조，차이균유통계학의의（P＜0．01）；이B림파세포、NK세포백분솔차이균무통계학의의（P＞0．05）；단순 HIV조화신경계통HIV조간뇌척액각림파세포아군백분솔차이무통계학의의（P＞0．05）。결론 HIV감염자재조기미발생신경계통병발증시취출현뇌척액면역공능문란，림파세포아군변화추세여외주혈중일치，T 림파세포아군가능여H IV신경계통증상상관。
Objective To explore the lymphcyte subsets distribution in cerebrospinal fluid in patients with HIV infection ,and to investigate the clinical significance of the lymphocyte subsets in cerebrospinal fluid in HIV central nervous system complication . Methods 34 patients with HIV infection ,including 20 patients without nervous system symptoms (simple HIV group) and 14 pa‐tients with nervous system symptoms (neurological HIV group) ,and 15 cases of healthy people (control group) were selected . Flow cytometry was used to detect lymphocyte subsets ,and immunoturbidimetry was used to detect the level of IgG in cerebrospinal fluid .Results The percentage of CD8+ T cells was higher and percentage of CD4+ T cells was lower in the simple HIV group and neurological HIV group than those in the healthy control ,with statistically significant differences (P<0 .01) .The level of IgG in pa‐tients with HIV infection was higher than that in the healthy control group (P<0 .01) .While no significant difference were found in the percentage of B cells and NK cells among the there group (P>0 .05) .There were also no significant differece between the sim‐ple HIV group and neurological HIV group in the ratio of each lymphcyte subset in cerebrospinal fluid (P>0 .05) .Conclusion The immune disorder in cerebrospinal fluid in patients with HIV infection may appear in the early time before the nervous system com‐plication .The changing trends of lymphocyte subsets are consistent with the peripheral blood ,which demonstrate that the T lym‐phocyte subsets may be correlated with the nervous system symptoms of HIV .