CAJ | 학술논문

目的：：探讨纤连蛋白( fibronectin,FN)降解片段对心肌细胞腱糖蛋白C( tenascin-C,TN-C)再表达的影响。方法：通过构建携带FN片段(包括FN-f1、FN-f2、FN-f3、FN-f4、FN-f5、FN-f6和FN-f7)慢病毒载体转染H9C2心肌细胞,采用免疫荧光及Western blotting检测TN-C的表达和分布情况。结果：相比于对照组,FN-f1、FN-f2、FN-f3和FN-f5组,免疫荧光结果显示TN-C表达升高幅度分别为(2．72±0．11)、(2．51±0．06)、(2．14±0．10)、(2．39±0．09), Western blotting则分别升高(0．415±0．009)、(0．290±0．012)、(0．386±0．014)、(0．332±0．016),TN-C表达显著升高,同时FN表达不受影响。而FN-f4、FN-f6和FN-f7对TN-C及FN的表达均没有影响。结论：部分FN片段可能参与TN-C的再表达和心肌重构的过程。
목적：：탐토섬련단백( fibronectin,FN)강해편단대심기세포건당단백C( tenascin-C,TN-C)재표체적영향。방법：통과구건휴대FN편단(포괄FN-f1、FN-f2、FN-f3、FN-f4、FN-f5、FN-f6화FN-f7)만병독재체전염H9C2심기세포,채용면역형광급Western blotting검측TN-C적표체화분포정황。결과：상비우대조조,FN-f1、FN-f2、FN-f3화FN-f5조,면역형광결과현시TN-C표체승고폭도분별위(2．72±0．11)、(2．51±0．06)、(2．14±0．10)、(2．39±0．09), Western blotting칙분별승고(0．415±0．009)、(0．290±0．012)、(0．386±0．014)、(0．332±0．016),TN-C표체현저승고,동시FN표체불수영향。이FN-f4、FN-f6화FN-f7대TN-C급FN적표체균몰유영향。결론：부분FN편단가능삼여TN-C적재표체화심기중구적과정。
Objective: To determine which fibronectin ( FN) degradation fragments contribute to the induction of tenascin-C ( TN-C) re-expression. Methods:Lentiviral vectors carrying FN fragments,including FN-f1,FN-f2,FN-f3,FN-f4,FN-f5,FN-f6 and FN-f7 were constructed. The protein expressions of TN-C and FN were measured with immunofluorescence assay and Western blotting. Re-sults:Transfection with FN-f1,FN-f2,FN-f3 and FN-f5 significantly induced TN-C expression but did not affect FN expression in H9C2 cells. However,FN-f4,FN-f6 and FN-f7 did not affect the TN-C expression. Conclusion:Some FN fragments may be involved in TN-C re-expression and the development of ventricular remodeling.