检验医学与临床
檢驗醫學與臨床
검험의학여림상
JOURNAL OF LABORATORY MEDICINE AND CLINICAL SCIENCES
2015年
9期
1268-1270
,共3页
血型%筛查%血型分析仪
血型%篩查%血型分析儀
혈형%사사%혈형분석의
blood types%screening%blood analyzer
目的：探讨并评价全自动血型分析仪应用于血站献血者血型筛查的可行性。方法采用全自动血型分析仪（全自动法）对20335例献血者标本进行ABO及Rh（D）血型鉴定、盐水不规则抗体初筛，并与U 型微板法（半自动法）进行比对试验。结果全自动法与半自动法比较，ABO血型1次准确定型率：99．93％＞98．84％，差异无统计学意义（ P＞0．05）；Rh‐D血型鉴定正确率均为100．0％；O细胞凝集阳性率：0．13％＞0．06％，差异有统计学意义（ P＜0．05）；可保持反定型检测准确度达100．0％的血浆稀释倍数为1∶64，高于1∶4；脂血标本和溶血标本导致的误判率差异有统计学意义（P＜0．05）。结论全自动血型分析仪具有较高的准确度、灵敏度及抗干扰能力，更易发现盐水不规则抗体，适合于血站开展献血者血型筛查工作。
目的：探討併評價全自動血型分析儀應用于血站獻血者血型篩查的可行性。方法採用全自動血型分析儀（全自動法）對20335例獻血者標本進行ABO及Rh（D）血型鑒定、鹽水不規則抗體初篩，併與U 型微闆法（半自動法）進行比對試驗。結果全自動法與半自動法比較，ABO血型1次準確定型率：99．93％＞98．84％，差異無統計學意義（ P＞0．05）；Rh‐D血型鑒定正確率均為100．0％；O細胞凝集暘性率：0．13％＞0．06％，差異有統計學意義（ P＜0．05）；可保持反定型檢測準確度達100．0％的血漿稀釋倍數為1∶64，高于1∶4；脂血標本和溶血標本導緻的誤判率差異有統計學意義（P＜0．05）。結論全自動血型分析儀具有較高的準確度、靈敏度及抗榦擾能力，更易髮現鹽水不規則抗體，適閤于血站開展獻血者血型篩查工作。
목적：탐토병평개전자동혈형분석의응용우혈참헌혈자혈형사사적가행성。방법채용전자동혈형분석의（전자동법）대20335례헌혈자표본진행ABO급Rh（D）혈형감정、염수불규칙항체초사，병여U 형미판법（반자동법）진행비대시험。결과전자동법여반자동법비교，ABO혈형1차준학정형솔：99．93％＞98．84％，차이무통계학의의（ P＞0．05）；Rh‐D혈형감정정학솔균위100．0％；O세포응집양성솔：0．13％＞0．06％，차이유통계학의의（ P＜0．05）；가보지반정형검측준학도체100．0％적혈장희석배수위1∶64，고우1∶4；지혈표본화용혈표본도치적오판솔차이유통계학의의（P＜0．05）。결론전자동혈형분석의구유교고적준학도、령민도급항간우능력，경역발현염수불규칙항체，괄합우혈참개전헌혈자혈형사사공작。
Objective To research and evaluate the feasibility for applying the fully automatic blood analyzer in donators′blood type screening in blood station .Methods The fully automatic blood analyzer (automatic method) was adopted to conduct the ABO and Rh‐D blood typing and brine irregular antibody preliminary screening in 20 335 specimens of blood donors ,and then the detection results were compared with those detected by using the U micro‐plate method (semi‐automatic contrast test) .Results Comparing the fully automatic method with the semi‐automatic method ,the once accurate typing rate of ABO type:99 .93% >98 .84% ,the difference had no statistical significance (P>0 .05);the accuracy rate of Rh(D)type identification was 100 .0% ;the positive rate of O cell agglutination:0 . 13% >0 .06% ,the difference was statistically significant(P<0 .05);the plasma dilution ratio of keeping the reverse typing detection′s accuracy up to 100 .0% was 1∶64 ,higher than 1∶4;the difference in the misjudgment rate caused by hemolysis and lipemia blood specimens was statistically significant(P< 0 .01) .Conclusion The fully automatic blood analyzer has higher accuracy ,sensitivity and anti‐interference ability ,is easier to find the brine irregular anti‐bodies and suitable for the donors′blood type screening work in blood station .
