国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2015年
5期
321-324
,共4页
BCG-CpG-DNA%哮喘%信号转导和转录活化因子4%信号转导和转录活化因子6
BCG-CpG-DNA%哮喘%信號轉導和轉錄活化因子4%信號轉導和轉錄活化因子6
BCG-CpG-DNA%효천%신호전도화전록활화인자4%신호전도화전록활화인자6
BCG-CpG-DNA%Asthma%Signal transducers and activators of transcription 4%Signal transducers and activators of transcription 6
目的 观察BCG-CpG-DNA干预对支气管哮喘(简称哮喘)小鼠信号转导和转录激活因子4(STAT4)、STAT6的影响,探讨其对小鼠哮喘的作用机制.方法 将30只BALB/c小鼠随机分为3组:正常对照组(A组)、哮喘模型组(B组)、BCG-CpG-DNA治疗组(C组).其中B、C组小鼠于第0天、第13天分别给予卵清白蛋白(OVA)和佐剂液态铝混悬液致敏,于第25天至第30天每天给予雾化OVA建立哮喘模型,C组于致敏后以BCG-CpG-DNA腹腔注射,A组以生理盐水代替OVA致敏和激发.所有动物于第31天处死,采用Western blot技术检测小鼠肺组织中STAT4、STAT6的蛋白表达水平.结果 与A组比较,B组STAT4、pSTAT4表达降低(P值均＜0.01),STAT6、pSTAT6的表达升高(P值均＜0.01);与B组比较,BCG-CpG-DNA治疗后小鼠哮喘症状和肺组织的炎症病理变化减轻,STAT4、pSTAT4表达增加,STAT6、pSTAT6表达降低,差异具有统计学意义(P值均＜0.01).结论 BCG-CpG-DNA可能通过抑制STAT6、促进STAT4的表达,调节Th1/Th2细胞因子平衡,从而起到抑制气道炎症的作用.
目的 觀察BCG-CpG-DNA榦預對支氣管哮喘(簡稱哮喘)小鼠信號轉導和轉錄激活因子4(STAT4)、STAT6的影響,探討其對小鼠哮喘的作用機製.方法 將30隻BALB/c小鼠隨機分為3組:正常對照組(A組)、哮喘模型組(B組)、BCG-CpG-DNA治療組(C組).其中B、C組小鼠于第0天、第13天分彆給予卵清白蛋白(OVA)和佐劑液態鋁混懸液緻敏,于第25天至第30天每天給予霧化OVA建立哮喘模型,C組于緻敏後以BCG-CpG-DNA腹腔註射,A組以生理鹽水代替OVA緻敏和激髮.所有動物于第31天處死,採用Western blot技術檢測小鼠肺組織中STAT4、STAT6的蛋白錶達水平.結果 與A組比較,B組STAT4、pSTAT4錶達降低(P值均＜0.01),STAT6、pSTAT6的錶達升高(P值均＜0.01);與B組比較,BCG-CpG-DNA治療後小鼠哮喘癥狀和肺組織的炎癥病理變化減輕,STAT4、pSTAT4錶達增加,STAT6、pSTAT6錶達降低,差異具有統計學意義(P值均＜0.01).結論 BCG-CpG-DNA可能通過抑製STAT6、促進STAT4的錶達,調節Th1/Th2細胞因子平衡,從而起到抑製氣道炎癥的作用.
목적 관찰BCG-CpG-DNA간예대지기관효천(간칭효천)소서신호전도화전록격활인자4(STAT4)、STAT6적영향,탐토기대소서효천적작용궤제.방법 장30지BALB/c소서수궤분위3조:정상대조조(A조)、효천모형조(B조)、BCG-CpG-DNA치료조(C조).기중B、C조소서우제0천、제13천분별급여란청백단백(OVA)화좌제액태려혼현액치민,우제25천지제30천매천급여무화OVA건립효천모형,C조우치민후이BCG-CpG-DNA복강주사,A조이생리염수대체OVA치민화격발.소유동물우제31천처사,채용Western blot기술검측소서폐조직중STAT4、STAT6적단백표체수평.결과 여A조비교,B조STAT4、pSTAT4표체강저(P치균＜0.01),STAT6、pSTAT6적표체승고(P치균＜0.01);여B조비교,BCG-CpG-DNA치료후소서효천증상화폐조직적염증병리변화감경,STAT4、pSTAT4표체증가,STAT6、pSTAT6표체강저,차이구유통계학의의(P치균＜0.01).결론 BCG-CpG-DNA가능통과억제STAT6、촉진STAT4적표체,조절Th1/Th2세포인자평형,종이기도억제기도염증적작용.
Objective To observe the influence of BCG-CpG-DNA on expression of signal transducers and activators of transcription 4 (STAT4),STAT6 in asthmatic mice lung,in order to discuss the potential mechanism of BCG-CpG-DNA in treating bronchial asthma (asthma).Methods Thirty BALB/c mice were randomly divided into three groups:normal control group (group A) and asthma model group (group B),BCG-CpG-DNA treated group (group C).Asthma model was established by OVA.Mice in group B and C were treated with mixed suspension of OVA and liquid aluminum adjuvant at day 0 and 13 respectively,and given aerosolized OVA from day 25 to 30,once every day.Mice in group C were intraperitoneal injected BCG-CpG-DNA after sensitization by OVA.OVA was replaced by physiological saline in group A.All the animals were killed at day 31.Western blot was performed to detect the protein expression level of STAT4 and STAT6 in the lung tissues of different group mice.Results The expression of STAT4 and pSTAT4 in group B were lower than group A (P ＜0.01).The expression of STAT6 and pSTAT6 in group B were higher than group A (P ＜0.01).BCG-CpG-DNA relieved the symptoms of asthma,increased the expression level of STAT4 and pSTAT4,depressed the the expression level of STAT6 and pSTAT6 when compared with group B.Differences are statistically significant (P ＜0.01).Conclusions BCG-CpG-DNA may regulate the cytokines balance of Th1 and Th2 by inhibiting STAT6 and promoting STAT4 to adjust the airway allergic inflammation.
