CAJ | 학술논문

目的：探讨转化生长因子（TGF）‐β1血浆水平及TGF‐β1基因－509C／T单核苷酸多态性（SNP）与重庆地区汉族人群经皮冠状动脉介入治疗术后支架内再狭窄（ISR）发生的关系。方法回顾性纳入冠状动脉支架术后行冠状动脉造影随访的患者368例，根据复查造影的结果将其分为IS R组152例和无再狭窄（N IS R ）组216例。酶联免疫吸附试验检测血浆TGF‐β1水平，采用聚合酶链反应‐限制片段长度多态性及基因测序的方法检测 TGF‐β1基因－509C／T多态的基因型。结果 TGF‐β1基因－509C／T多态的3种基因型和等位基因分布频率ISR组和NISR组差异均有统计学意义（P＜0．05），TT基因型和T等位基因在ISR组所占比例显著增加，与NISR组差异有统计学意义（P＜0．05）；血浆TGF‐β1水平ISR组高于NISR组，差异有统计学意义（P＜0．05），ISR组TGF‐β1基因－509C／T多态TT和CT基因型携带者血浆TGF‐β1水平均显著高于NISR组，差异有统计学意义（P＜0．05），各组内TT基因型血浆TGF‐β1水平均高于CC和CT基因型，CT基因型又高于CC基因型，组内比较差异均有统计学意义（P＜0．05）。Logistic回归分析显示，TT基因型、T等位基因（CT＋ TT基因型）和血浆TGF‐β1是ISR发生的独立危险因素（OR值分别为1．82、1．61和2．01，P＜0．05）。结论高血浆TGF‐β1水平、TT基因型及T等位基因携带者显著增加IS R的风险。
목적：탐토전화생장인자（TGF）‐β1혈장수평급TGF‐β1기인－509C／T단핵감산다태성（SNP）여중경지구한족인군경피관상동맥개입치료술후지가내재협착（ISR）발생적관계。방법회고성납입관상동맥지가술후행관상동맥조영수방적환자368례，근거복사조영적결과장기분위IS R조152례화무재협착（N IS R ）조216례。매련면역흡부시험검측혈장TGF‐β1수평，채용취합매련반응‐한제편단장도다태성급기인측서적방법검측 TGF‐β1기인－509C／T다태적기인형。결과 TGF‐β1기인－509C／T다태적3충기인형화등위기인분포빈솔ISR조화NISR조차이균유통계학의의（P＜0．05），TT기인형화T등위기인재ISR조소점비례현저증가，여NISR조차이유통계학의의（P＜0．05）；혈장TGF‐β1수평ISR조고우NISR조，차이유통계학의의（P＜0．05），ISR조TGF‐β1기인－509C／T다태TT화CT기인형휴대자혈장TGF‐β1수평균현저고우NISR조，차이유통계학의의（P＜0．05），각조내TT기인형혈장TGF‐β1수평균고우CC화CT기인형，CT기인형우고우CC기인형，조내비교차이균유통계학의의（P＜0．05）。Logistic회귀분석현시，TT기인형、T등위기인（CT＋ TT기인형）화혈장TGF‐β1시ISR발생적독립위험인소（OR치분별위1．82、1．61화2．01，P＜0．05）。결론고혈장TGF‐β1수평、TT기인형급T등위기인휴대자현저증가IS R적풍험。
Objective To explore the association between TGF‐β1 gene C‐509T single nucleotide polymor‐phism(SNP) and plasma levels of TGF‐β1 with the occurrence of coronary stent restenosis after percutaneous coro‐nary intervention(PCI) in Han nationality crowd .Methods 368 patients with successful coronary stent placement and follow‐up angiography were retrospectively included and divided into the in‐stent restenosis (ISR) group (n=152) and non in‐stent restenosis (NISR) group (n=216) .Genotyping for the C‐509T polymorphism was performed by using polymerase chain reaction‐restriction fragment length polymorphism analysis .Plasma TGF‐β1 levels were detected with the enzyme linked immuno sorbent assay(ELISA) .Results The prevalence of the TT genotype ,fre‐quency of the T allele and the concentration of TGF‐β1 in the ISR group were significantly higher than those in the NISR group .The C‐509T polymorphism was also significantly correlated with TGF‐β1 levels with the TT genotype corresponding with the highest and the CC genotype with the lowest TGF‐β1 levels in both ISR and NISR group .The TT genotype ,T allele carriers and plasma TGF‐β1 levels were significantly associated with the risk of ISR (adjusted OR=1 .82 ,1 .61 ,2 .01 ,P<0 .05) .Conclusion Higher plasma levels of TGF‐β1 ,T T homozygotes and T allele carri‐ers of TGF‐β1 C‐509T polymorphism have higher risk of ISR after coronary stenting .