CAJ | 학술논문

目的：探讨大鼠创伤性脑损伤后17β雌二醇对脑组织的保护作用。方法选择雄性成年 SD大鼠45只，按随机数字表法分为三组，每组15只：对照组仅开骨窗，不损伤脑组织；致伤组制大鼠自由落体脑撞击伤模型；处理组在致伤组的基础上，伤前1周腹腔注射17β雌二醇溶液（1 mg／kg），1次／d。余两组仅注射同体积的蓖麻油。在伤后6 h、24 h及48 h测量各组脑组织含水量、丙二醛（ MDA）含量及超氧化物歧化酶（ SOD）活性。结果致伤组和处理组在伤后6 h、24 h、48 h三个时间点脑组织含水量均高于对照组（P＜0．05）。在伤后6 h，致伤组和处理组脑组织含水量比较差异无统计学意义[（58．39±0．29）％比（57．03±0．27）％]（ P＞0．05 ），而在伤后24 h、48 h 致伤组脑组织含水量明显高于处理组，差异有统计学意义[（67．41±0．37）％比（64．77±0．33）％，（81．95±0．47）％比（75．26±0．41）％]（ P＜0．05）。在伤后6 h致伤组、处理组MDA含量明显增加，并且一直维持在较高水平，而SOD活性则明显下降，与对照组比较差异有统计学意义（ P＜0．05）。处理组在伤后6 h MDA含量和SOD活性与致伤组比较差异无统计学意义（P＞0．05），而在伤后24 h、48 h MDA 含量显著降低[（130．39±7．02）μmol／g比（149．41±8．25）μmol／g，（125．41±6．59）μmol／g 比（157．72±8．93）μmol／g]， SOD活性则明显增高[（88．46±7．17）U／g比（80．10±4．87）U／g，（97．31±7．89）U／g比（84．29±6．13） U／g]，差异均有统计学意义（ P＜0．05）。结论17β雌二醇对创伤性脑损伤有保护作用。
목적：탐토대서창상성뇌손상후17β자이순대뇌조직적보호작용。방법선택웅성성년 SD대서45지，안수궤수자표법분위삼조，매조15지：대조조부개골창，불손상뇌조직；치상조제대서자유락체뇌당격상모형；처리조재치상조적기출상，상전1주복강주사17β자이순용액（1 mg／kg），1차／d。여량조부주사동체적적비마유。재상후6 h、24 h급48 h측량각조뇌조직함수량、병이철（ MDA）함량급초양화물기화매（ SOD）활성。결과치상조화처리조재상후6 h、24 h、48 h삼개시간점뇌조직함수량균고우대조조（P＜0．05）。재상후6 h，치상조화처리조뇌조직함수량비교차이무통계학의의[（58．39±0．29）％비（57．03±0．27）％]（ P＞0．05 ），이재상후24 h、48 h 치상조뇌조직함수량명현고우처리조，차이유통계학의의[（67．41±0．37）％비（64．77±0．33）％，（81．95±0．47）％비（75．26±0．41）％]（ P＜0．05）。재상후6 h치상조、처리조MDA함량명현증가，병차일직유지재교고수평，이SOD활성칙명현하강，여대조조비교차이유통계학의의（ P＜0．05）。처리조재상후6 h MDA함량화SOD활성여치상조비교차이무통계학의의（P＞0．05），이재상후24 h、48 h MDA 함량현저강저[（130．39±7．02）μmol／g비（149．41±8．25）μmol／g，（125．41±6．59）μmol／g 비（157．72±8．93）μmol／g]， SOD활성칙명현증고[（88．46±7．17）U／g비（80．10±4．87）U／g，（97．31±7．89）U／g비（84．29±6．13） U／g]，차이균유통계학의의（ P＜0．05）。결론17β자이순대창상성뇌손상유보호작용。
Objective To explore the protective effect of 17β-estradiol on traumatic brain injury in rats.Methods A total of 45 adult male SD rats were divided into 3 groups using the random digit table, 15 rats in each group:the control group only exposed but not injured the brain, the injury group received traumatic brain injury ( TBI) by Feeney’ s method, and the treatment group received the same handling with injury group, and pretreatment with 17β-estradiol peritoneal injection, 1 mg/kg per day for one week.The other two groups were given the same volume of castor oil.At 6 h, 24 h and 48 h after injury, the brain tissue water content, malondialdehyde ( MDA) content and superoxide dismutase ( SOD) activity were measured.Results At 6 h, 24 h and 48 h after injury, the levels of brain tissue water content in the injury group and treatment group were significantly higher than those in the control group ( P<0.05 ) .At 6 h after injury, the brain tissue water contents in the injury group and treatment group were ( 99.83 ±0.40 )% and ( 99.53 ±0.41 )%,respectively, with a non-significant difference between the two groups (P>0.05).At 24 h and 48 h after injury, the brain tissue water contents in the injury group was ( 105.17 ±0.43 )% and ( 107.54 ±0.39 )%, in the treatment group was (103.26 ±0.42)%and (105.89 ±0.43)%, respectively, showing a significant difference between the two groups ( P<0.05).At 6 h after injury, the levels of MDA in the injury group and treatment group increased and maintained at a higher level, and the levels of SOD decreased.Compared with the control group, there had a significant difference ( P<0.05 ) . The levels of MDA and SOD in the injury group and treatment group at 6 h after injury had a non-significant difference ( P>0.05).But at 24 h and 48 h after injury, the levels of MDA in the treatment group were significantly lower than those in the injury group [(130.39 ±7.02) μmol/g vs.(149.41 ±8.25) μmol/g, (125.41 ±6.59) μmol/g vs.(157.72 ± 8.93) μmol/L] , and the levels of SOD in the treatment group were significantly higher than thoset in the injury group [(88.46 ±7.17) U/g vs.(80.10 ±4.87) U/mg, (97.31 ±7.89) U/g vs.(84.29 ±6.13) U/g], with a significant difference ( P<0.05 ) between the two groups.Conclusions 17β-estradiol has a protective effect on traumatic brain injury.