CAJ | 학술논문

目的：观察急性血液稀释技术（ ANH）在狗断肢冷冻再植实验中对改善术中、术后动物全身状态的作用。方法在狗后肢深低温冷冻再植实验中，对照组（A组）采用静脉补充等量乳酸钠林格氏液，实验组（B组）采用急性血液稀释技术，对比两组各时点循环动力学指标、血常规、术后动物苏醒时间、抬头时间、站立时间、术后进食、进水时间。结果①术中两组PaO2、PCO2值均在正常范围，呼吸频率正常，B组心率明显低于A组，术中血红蛋白、红细胞压积输血前A组与B组无统计学差异，术后B组明显高于A组，差异存在显著性。②术后动物苏醒时间、抬头时间、站立时间、术后进食量、进水量B组均明显优于A组，存在显著差异。结论在狗断肢深低温冷冻再植实验中，急性血液稀释技术（ ANH）能明显改善动物术中的全身状态，加快术后恢复速度。
목적：관찰급성혈액희석기술（ ANH）재구단지냉동재식실험중대개선술중、술후동물전신상태적작용。방법재구후지심저온냉동재식실험중，대조조（A조）채용정맥보충등량유산납림격씨액，실험조（B조）채용급성혈액희석기술，대비량조각시점순배동역학지표、혈상규、술후동물소성시간、태두시간、참립시간、술후진식、진수시간。결과①술중량조PaO2、PCO2치균재정상범위，호흡빈솔정상，B조심솔명현저우A조，술중혈홍단백、홍세포압적수혈전A조여B조무통계학차이，술후B조명현고우A조，차이존재현저성。②술후동물소성시간、태두시간、참립시간、술후진식량、진수량B조균명현우우A조，존재현저차이。결론재구단지심저온냉동재식실험중，급성혈액희석기술（ ANH）능명현개선동물술중적전신상태，가쾌술후회복속도。
Objective To observe the contribution of acute normovolemic hemodilution ( ANH) in experiment of cryopreserved dog limb replantation.Methods Sixteen healthy Beagle dogs (male:female=1:1) were divided into two groups.Dogs in the experiment group ( Group B) received ANH in the limb replantation, and dogs in the control group ( Group A) received the same amount of lactate Ringer’ s solution intravenously during the surgical operation.We recorded and compared the hemodynamic indexes, HB, HCT, the resuscitation time, the first rising head time, the first standing time and the first eating and drinking time between the two groups.Results ( 1 ) During the operation, both PaO2 and PCO2 in the two groups were normal, as well as the breathing rate.The heart rate in the group B was lower than that in the group A.Before blood transfusion, there was no statistically significant difference in HB and HCT between the two groups, but after transfusion they were significantly higher in the group B than in the group A.(2) The resuscitation time, the first rising head time, the first standing time and the first eating and drinking time of the group B were all better than those in the group A.Conclusions In cryopreserved dog limb replantation experiments, acute normovolemic hemodilution is helpful to improve the general condition and facilitate the recovery of animals after limb replantation.