CAJ | 학술논문

目的：研究人参皂苷水解产物DS－1227（ Dammarane Sapogenins－1227）对东莨菪碱致小鼠学习记忆障碍的改善作用，为老年痴呆等疾病的药物研究提供科学数据。方法将60只ICR小鼠随机分为空白对照组、模型组、阳性对照组、DS－1227低、中、高剂量组共6组，每组10只。连续灌胃给药2周后，检测自主活动，第15天开始除空白对照组腹腔注射生理盐水，其他各组均腹腔注射东莨菪碱（0．75 mg／kg ），阳性对照组在注射东莨菪碱5 min后另腹腔注射毒扁豆碱（0．3 mg／kg），给药完成20 min后进行物体认知实验和Morris水迷宫实验检测动物的学习记忆能力。结果 DS－1227对小鼠的自主活动无显著性影响。腹腔注射东莨菪碱后，小鼠的相对辨别指数降低，水迷宫定位航行实验中d1、d2、d3、d4和d5潜伏期均明显延长。 DS－1227低、中、高剂量和给予M受体激动剂毒扁豆碱均能不同程度增加东莨菪碱致学习记忆障碍模型小鼠的相对辨别指数，缩短水迷宫定位航行潜伏期。结论DS－1227有改善东莨菪碱致小鼠学习记忆障碍的作用。
목적：연구인삼조감수해산물DS－1227（ Dammarane Sapogenins－1227）대동랑탕감치소서학습기억장애적개선작용，위노년치태등질병적약물연구제공과학수거。방법장60지ICR소서수궤분위공백대조조、모형조、양성대조조、DS－1227저、중、고제량조공6조，매조10지。련속관위급약2주후，검측자주활동，제15천개시제공백대조조복강주사생리염수，기타각조균복강주사동랑탕감（0．75 mg／kg ），양성대조조재주사동랑탕감5 min후령복강주사독편두감（0．3 mg／kg），급약완성20 min후진행물체인지실험화Morris수미궁실험검측동물적학습기억능력。결과 DS－1227대소서적자주활동무현저성영향。복강주사동랑탕감후，소서적상대변별지수강저，수미궁정위항행실험중d1、d2、d3、d4화d5잠복기균명현연장。 DS－1227저、중、고제량화급여M수체격동제독편두감균능불동정도증가동랑탕감치학습기억장애모형소서적상대변별지수，축단수미궁정위항행잠복기。결론DS－1227유개선동랑탕감치소서학습기억장애적작용。
Objective The aim of this experiment was to study the improving effects of a ginsenoside hydrolysis product DS-1227 on scopolamine-induced learning and memory impairment in mice.Methods Sixty healthy 5-6-week old male ICR mice (body weight 22 ±2 g) were randomly divided into control group, model group, three DS-1227 groups (25 mg/kg, 50 mg/kg, 100 mg/kg), and positive control group (0.3 mg/kg).Fourteen days after oral administration of DS-1227, an open-field test was conduct to determine the mouse locomotor activity.Fifteen days after oral administration of DS-1227, all experimental animals were intraperitoneally administered scopolamine (0.75 mg/kg) and the mice of control group received the same volume of saline.In addition to scopolamine, the mice of positive control group received intraperitoneal injection of physostigmine in a dose of 0.3 mg/kg.Twenty minutes after completion of all the drug administration, object recognition test and Morris water maze test were conducted to evaluate the learning and memory abilities of the mice.Results DS-1227 had no significant effect on locomotor activity of the mice.Scopolamine obviously decreased the discrimination indexes in object recognition test, and prolonged the escape latency of water maze place navigation test.While 25 mg/kg, 50 mg/kg, 100 mg/kg of DS-1227 increased the discrimination indexes and decreased the escape latency of place navigation in the mice.Conclusion DS-1227 can improve the learning and memory impairment induced by scopolamine in mice.