CAJ | 학술논문

背景：克罗恩病（CD）的发病机制与机体免疫应答异常有关，T 细胞参与机体免疫应答过程。目的：研究 CD 患者外周血 CD3＋、CD4＋、CD8＋ T 细胞变化与炎症相关指标的关系。方法：应用流式细胞仪检测26例 CD 患者（活动期14例和缓解期12例）和8名健康志愿者（对照组）外周血 CD3＋、CD4＋、CD8＋ T 细胞比例，并分析与炎症相关指标（白细胞计数、血小板计数、ESR、CRP、白蛋白和血红蛋白）的相关性。结果：CD 活动期 CD3＋、CD4＋、CD8＋ T 细胞比例均明显高于缓解期和对照组（P ﹤0.05），但缓解期与对照组间差异无统计学意义（ P ﹥0.05）。CD 活动期ESR 和 CRP 明显高于对照组（P ﹤0.05），血红蛋白和白蛋白水平明显低于对照组（P ﹤0.05），CD 缓解期白蛋白水平明显低于对照组（P ﹤0.05），而 CD 活动期和缓解期白细胞计数、血小板计数与对照组相比差异均无统计学意义（P ﹥0.05）。CD3＋、CD4＋和 CD8＋ T 细胞比例与 CRP 呈正相关、与血红蛋白呈负相关（P ﹤0.05），CD3＋、CD4＋ T 细胞比例与 ESR 呈正相关（P ﹤0.05）；CD3＋、CD4＋和 CD8＋ T 细胞比例与白细胞、血小板计数和白蛋白均无关（P ﹥0.05）。结论：CD 患者 CD3＋、CD4＋和 CD8＋ T 细胞比例随病情活动而升高，并与 CRP 呈正相关，与血红蛋白呈负相关。
배경：극라은병（CD）적발병궤제여궤체면역응답이상유관，T 세포삼여궤체면역응답과정。목적：연구 CD 환자외주혈 CD3＋、CD4＋、CD8＋ T 세포변화여염증상관지표적관계。방법：응용류식세포의검측26례 CD 환자（활동기14례화완해기12례）화8명건강지원자（대조조）외주혈 CD3＋、CD4＋、CD8＋ T 세포비례，병분석여염증상관지표（백세포계수、혈소판계수、ESR、CRP、백단백화혈홍단백）적상관성。결과：CD 활동기 CD3＋、CD4＋、CD8＋ T 세포비례균명현고우완해기화대조조（P ﹤0.05），단완해기여대조조간차이무통계학의의（ P ﹥0.05）。CD 활동기ESR 화 CRP 명현고우대조조（P ﹤0.05），혈홍단백화백단백수평명현저우대조조（P ﹤0.05），CD 완해기백단백수평명현저우대조조（P ﹤0.05），이 CD 활동기화완해기백세포계수、혈소판계수여대조조상비차이균무통계학의의（P ﹥0.05）。CD3＋、CD4＋화 CD8＋ T 세포비례여 CRP 정정상관、여혈홍단백정부상관（P ﹤0.05），CD3＋、CD4＋ T 세포비례여 ESR 정정상관（P ﹤0.05）；CD3＋、CD4＋화 CD8＋ T 세포비례여백세포、혈소판계수화백단백균무관（P ﹥0.05）。결론：CD 환자 CD3＋、CD4＋화 CD8＋ T 세포비례수병정활동이승고，병여 CRP 정정상관，여혈홍단백정부상관。
Background:Abnormal immune response is involved in the pathogenesis of Crohn’s disease( CD),and T lymphocytes are the main players in the immune response. Aims:To investigate the relationship between peripheral blood CD3 + ,CD4 + and CD8 + T cells and inflammation-related markers in patients with CD. Methods:Proportions of peripheral blood CD3 + ,CD4 + and CD8 + T cells were measured by flow cytometry in 26 CD patients( including 14 patients in active stage and 12 in remission stage )and 8 healthy volunteers(control group),and their correlation with inflammation-related markers(including white blood cell count,platelet count,ESR,CRP,albumin and hemoglobin) were analyzed. Results:Proportions of CD3 + ,CD4 + and CD8 + T cells were significantly increased in patients with active CD than those with remission CD and controls( P ﹤ 0. 05),however,no significant differences were found between remission CD patients and controls(P ﹥ 0. 05). ESR and CRP in active CD patients were significantly higher than those in controls(P ﹤ 0. 05),while albumin and hemoglobin levels were significantly decreased(P ﹤ 0. 05);albumin in remission CD patients was significantly lower than that in controls(P ﹤ 0. 05). No significant differences in white blood cell count and platelet count were found between active,remission CD patients and controls(P ﹥ 0. 05). Proportions of CD3 + , CD4 + and CD8 + T cells were positively correlated with CRP,and negatively correlated with hemoglobin( P ﹤ 0. 05);CD3 + and CD4 + T cells were positively correlated with ESR(P ﹤ 0. 05). However,CD3 + ,CD4 + and CD8 + T cells were not correlated with white blood cell count,platelet count and albumin level( P ﹥ 0. 05). Conclusions:Proportions of peripheral blood CD3 + ,CD4 + and CD8 + T cells are increased with the increase of disease activity in CD,and are positively correlated with CRP,and negatively correlated with hemoglobin.