CAJ | 학술논문

目的脑源性神经营养因子前体蛋白(proBDNF)分布于中枢神经系统,在情绪障碍和神经退行性病变中起重要作用.为了深入理解proBDNF结构与功能的关系.方法从NCBI下载了人类proBDNF基因序列,并对基因及其编码的氨基酸序列进行生物信息学分析.结果序列分析结果显示,该基因编码247个氨基酸序列多肽,预测蛋白质相对分子质量为27.82×103,理论等电点为9.01.信号序列分析结果发现,该蛋白主要定位于细胞的内质网、溶酶体及细胞基质中,提示为细胞内蛋白及分泌性蛋白两种存在形式.结论结构与功能分析发现,该蛋白含有1个结构域及1个信号肽,推测proBDNF在真核细胞的信号转导、转录调控及胁迫表达等过程中发挥重要功能.
목적 뇌원성신경영양인자전체단백(proBDNF)분포우중추신경계통,재정서장애화신경퇴행성병변중기중요작용.위료심입리해proBDNF결구여공능적관계.방법 종NCBI하재료인류proBDNF기인서렬,병대기인급기편마적안기산서렬진행생물신식학분석.결과 서렬분석결과현시,해기인편마247개안기산서렬다태,예측단백질상대분자질량위27.82×103,이론등전점위9.01.신호서렬분석결과발현,해단백주요정위우세포적내질망、용매체급세포기질중,제시위세포내단백급분비성단백량충존재형식.결론 결구여공능분석발현,해단백함유1개결구역급1개신호태,추측proBDNF재진핵세포적신호전도、전록조공급협박표체등과정중발휘중요공능.
Objective Precursor of brain-derived neurotrophic factor (proBDNF),mainly located in the central nervous system,plays an important role in the process of emotional disorders and neurodegenerative disease.To investigate the relationship between the structure and function of proBDNF.Methods We downloaded the human genome sequence of proBDNF from NCBI genbank and analysed the gene and protein sequence of the proBDNF with bioinformatics technology.Results The results of proBDNF gene sequence analysis indicated that it encoded a protein composed of 247 amino acid residues,whose predicted molecular weight was 27.82 × 103 and theroretical isoelectric point was 9.01.Moreover,the results indicated that proBDNF distributed mainly in endomembrane system such as endoplasmic reticulum,lysosome and cell matix.existed in the form of endocellular and secretory protein.Conclusion The results of three-dimensional structure analysis revealed that proBDNF protein was composed of one structural domain and one signal peptide.It was speculated that proBDNF could play a critical role in signal transduction,regulation of transcription and stress-induced expression of eukaryotic cell.