CAJ | 학술논문

孕妇外周血中无细胞胎儿DNA(cffDNA)是无创性产前诊断中重要的胎儿物质的检测来源.由于孕妇血中大部分是母体DNA,而游离胎儿DNA的量非常少,仅占3％～6％.因此从孕妇血中成功分离cffDNA,对后续的无创性产前诊断有着十分重要的意义.本文分别从孕妇外周血中cffDNA的发现来源,cffDNA的结构与稳定性,分离孕妇外周血中的cffDNA的实验方法,及在无创性产前诊断中的应用等方面进行介绍,并着重对该技术近年来的研究进展作一综述.旨在探寻较高效率分离孕妇外周血中cffDNA的实验方法,为无创性产前诊断提供较高浓度的检测物质,提高其准确率及成功率.
잉부외주혈중무세포태인DNA(cffDNA)시무창성산전진단중중요적태인물질적검측래원.유우잉부혈중대부분시모체DNA,이유리태인DNA적량비상소,부점3％～6％.인차종잉부혈중성공분리cffDNA,대후속적무창성산전진단유착십분중요적의의.본문분별종잉부외주혈중cffDNA적발현래원,cffDNA적결구여은정성,분리잉부외주혈중적cffDNA적실험방법,급재무창성산전진단중적응용등방면진행개소,병착중대해기술근년래적연구진전작일종술.지재탐심교고효솔분리잉부외주혈중cffDNA적실험방법,위무창성산전진단제공교고농도적검측물질,제고기준학솔급성공솔.
Cell free fetal DNA (cffDNA) in maternal peripheral blood is an important material of fetus for detection in non-invasive prenatal diagnosis (NIPD).Most DNAs in maternal blood is maternal origin,the proportion of cffDNA is just 3 ％～ 6 ％.Therefore,successful isolation of cffDNA from maternal peripheral blood plays an important role in the subsequent NIPT.In this paper we will review the discovery,origin,structure and stability of cffDNA laboratory method of isolation from maternal blood,and the its application in NIPT,and emphasize on the recently research progress of technology systematically.The purpose is to explore the laboratory method for cffDNA isolation with high efficiency,to provide a high concentration of cffDNA for NIPT and increase the accuracy rate and success rate in NIPD.