CAJ | 학술논문

目的评价氢氧化钙[Ca(OH)2]溶液预处理对羧基改性的聚酰胺-胺型树枝状大分子(polyamidoamine dendrime,PAMAM)在脱矿牙本质再矿化中的影响,为治疗牙本质早期龋提供依据.方法制备32个脱矿人牙本质样本并分为4个组(每组8个):空白对照组未经任何处理;Ca(OH)2组使用Ca(OH)2溶液进行预处理;PAMAM组使用羧基改性的PAMAM溶液处理;PAMAM+Ca(OH)2组用羧基改性的PAMAM溶液处理后采用Ca(OH)2溶液预处理.之后各组牙本质样本在人工唾液中矿化2周.利用扫描电镜、能谱分析、X衍射分析评估4组牙本质样本再矿化效果.结果扫描电镜示空白对照组牙本质小管内无新生物,Ca(OH)2组牙本质小管有少量新生物,PAMAM组部分牙本质小管内被新生物封闭,PAMAM+ Ca(OH)2组再矿化程度最佳,几乎所有牙本质小管均被再矿化物封闭并呈半球形突出于表面.能谱分析和X射线衍射检测证实矿化物为类羟基磷灰石晶体.结论羧基改性的PAMAM协同Ca(OH)2溶液预处理可促进脱矿牙本质的再矿化,在治疗牙本质早期龋方面有良好的应用前景.
목적 평개경양화개[Ca(OH)2]용액예처리대최기개성적취선알-알형수지상대분자(polyamidoamine dendrime,PAMAM)재탈광아본질재광화중적영향,위치료아본질조기우제공의거.방법 제비32개탈광인아본질양본병분위4개조(매조8개):공백대조조미경임하처리;Ca(OH)2조사용Ca(OH)2용액진행예처리;PAMAM조사용최기개성적PAMAM용액처리;PAMAM+Ca(OH)2조용최기개성적PAMAM용액처리후채용Ca(OH)2용액예처리.지후각조아본질양본재인공타액중광화2주.이용소묘전경、능보분석、X연사분석평고4조아본질양본재광화효과.결과 소묘전경시공백대조조아본질소관내무신생물,Ca(OH)2조아본질소관유소량신생물,PAMAM조부분아본질소관내피신생물봉폐,PAMAM+ Ca(OH)2조재광화정도최가,궤호소유아본질소관균피재광화물봉폐병정반구형돌출우표면.능보분석화X사선연사검측증실광화물위류간기린회석정체.결론 최기개성적PAMAM협동Ca(OH)2용액예처리가촉진탈광아본질적재광화,재치료아본질조기우방면유량호적응용전경.
Objective To evaluate the remineralization effect of Ca(OH)2,pre-treated with carboxylmodified polyamidoamine dendrimer(PAMAM) on demineralized dentin.Methods Thirty-two demineralized dentin models were divided into four groups randomly as follows:control group with no treatment,Ca(OH)2 group pretreated with Ca(OH)2 solution,PAMAM group processed with carboxyl-modified PAMAM,and PAMAM + Ca(OH) 2 group processed with carboxyl-modified PAMAM and pretreated with Ca(OH)2 solution.All samples were immersed in artificial saliva for two weeks.The remineralization effects of dentin discs were evaluated by scanning electron microscope(SEM),energy dispersive spectrometer(EDS) and X-ray diffraction(XRD).Results Observed by the SEM,it was showed that in PAMAM + Ca(OH)2 group almost all the dentinal tubules were occluded by the minerals,however this was not found in other groups.The minerals proved were hydroxyapatite through EDS and XRD tests.Conclusions There was potential superiority of the carboxyl-modified PAMAM with Ca(OH)2 solution in promoting the remineralization of initial dentin lesions.