CAJ | 학술논문

目的：探讨职业性低水平接触1，3-丁二烯（BD）所致周围血淋巴细胞遗传损伤与DNA修复能力的相关性。方法采用横断面调查方法，以某石化企业60名BD作业人员为接触组，按照年龄和工龄1∶1匹配，以该企业无职业性接触BD的60名工作人员作为对照组。采用直接进样－气相色谱法测定工作场所空气中BD水平；采用胞质分裂阻滞微核试验和碱性彗星试验评价2组人群周围血淋巴细胞染色体和DNA损伤水平，采用博莱霉素（ BLM ）诱变剂敏感性试验评价周围血淋巴细胞对BLM所致DNA损伤的修复能力。结果接触组人群工作场所空气中BD的短时间接触浓度为0.59～2.76（1.80±0.20） mg／m3，对照组工作场所空气中未检出BD。接触组人群周围血双核淋巴细胞微核率、核质桥率、核芽率和Olive尾距均高于对照组[（1.81±0.96）‰ vs （1.02±0.68）‰，1.00‰（0.00～3.00）‰vs 0.00‰（0.00～1.00）‰，3.00‰（1.00～5.00）‰ vs 1.00‰（0.00～3.00）‰，5.25（3.96～5.73）μm vs 2.38（1.35～3.88）μm，P＜0.01]。接触组人群周围血淋巴细胞平均每细胞染色体断裂率（ b／c率）高于对照组[（1.06±0.41）％vs （0.85±0.36）％，P＜0.05]。对照组人群b／c率与微核率呈中度正相关[Spearman相关系数（rS）＝0.542，P＜0.01]，但均与核质桥率、核芽率和Olive尾距不相关（rS分别为0.213、0.273和－0.120， P＞0.05）；而接触组人群b／c率与微核率、核质桥率、核芽率均呈中度正相关（ rS 分别为0.730、0.515和0.660，P＜0.01），但与Olive尾距不相关（rS ＝－0.120，P＞0.05）。结论低水平BD可能导致职业接触人群周围血淋巴细胞遗传物质损伤，其与DNA修复能力可能存在一定的相关性。
목적：탐토직업성저수평접촉1，3-정이희（BD）소치주위혈림파세포유전손상여DNA수복능력적상관성。방법채용횡단면조사방법，이모석화기업60명BD작업인원위접촉조，안조년령화공령1∶1필배，이해기업무직업성접촉BD적60명공작인원작위대조조。채용직접진양－기상색보법측정공작장소공기중BD수평；채용포질분렬조체미핵시험화감성혜성시험평개2조인군주위혈림파세포염색체화DNA손상수평，채용박래매소（ BLM ）유변제민감성시험평개주위혈림파세포대BLM소치DNA손상적수복능력。결과접촉조인군공작장소공기중BD적단시간접촉농도위0.59～2.76（1.80±0.20） mg／m3，대조조공작장소공기중미검출BD。접촉조인군주위혈쌍핵림파세포미핵솔、핵질교솔、핵아솔화Olive미거균고우대조조[（1.81±0.96）‰ vs （1.02±0.68）‰，1.00‰（0.00～3.00）‰vs 0.00‰（0.00～1.00）‰，3.00‰（1.00～5.00）‰ vs 1.00‰（0.00～3.00）‰，5.25（3.96～5.73）μm vs 2.38（1.35～3.88）μm，P＜0.01]。접촉조인군주위혈림파세포평균매세포염색체단렬솔（ b／c솔）고우대조조[（1.06±0.41）％vs （0.85±0.36）％，P＜0.05]。대조조인군b／c솔여미핵솔정중도정상관[Spearman상관계수（rS）＝0.542，P＜0.01]，단균여핵질교솔、핵아솔화Olive미거불상관（rS분별위0.213、0.273화－0.120， P＞0.05）；이접촉조인군b／c솔여미핵솔、핵질교솔、핵아솔균정중도정상관（ rS 분별위0.730、0.515화0.660，P＜0.01），단여Olive미거불상관（rS ＝－0.120，P＞0.05）。결론저수평BD가능도치직업접촉인군주위혈림파세포유전물질손상，기여DNA수복능력가능존재일정적상관성。
Objective To explore the correlation between DNA repair capacity ( DRC) and genetic damage in peripheral blood lymphocytes of workers exposed to low level of 1,3-butadiene (BD).Methods With a cross-sectional study, 60 workers from a petrochemical enterprise who were exposed to BD were selected as the exposure group , and 1∶1 matched by age and length of service , 60 non-occupational BD exposure workers in the same enterprise were chosen as the control group.BD level in workplace air was measured by direct injection-gas chromatography determination method .Cytokinesis-block micronucleus ( MN) detection and alkaline comet assay were used to evaluate chromosomal and DNA damage levels in peripheral blood lymphocytes of the two groups .DRC for bleomycin ( BLM)-induced DNA damage in peripheral blood lymphocytes was measured by sensitivity test of the mutagenic agent BLM .Results The short time expose concentration of BD in the air of workplace of the exposure group was 0.59-2.76(1.80 ±0.20) mg/m3 , and BD was not detected in the air of workplace of the control group.The rates of MN, nucleoplasmic bridge (NPB), nuclear buds (NB) and Olive tail moment ( OTM ) of peripheral blood lymphocyte in the exposure group were higher than those in the control group respectively [(1.81 ±0.96)‰ vs (1.02 ±0.68)‰, 1.00‰(0.00-3.00)‰ vs 0.00‰(0.00-1.00)‰, 3.00‰(1.00-5.00)‰ vs 1.00‰(0.00-3.00)‰, 5.25(3.96-5.73) μm vs 2.38(1.35-3.88) μm, P <0.01].The chromatid breaksper cell (b/c rate) of peripheral blood lymphocyte in the exposure group was significantly higher than that in the controlgroup [(1.06 ±0.41)% vs (0.85 ±0.36)%, P <0.05].Among the control group, the b/c rates of peripheral bloodlymphocyte showed a statistical moderate positive correlation with the MN rate [Spearman correlation coefficient (rS ) was0.542, P <0.01], but not related to the NPB rate, NB rate and OTM respectively (rS were 0.213, 0.273 and -0.120, P >0.05).Among the exposure group, there was statistical moderate positive correlation between the b /c rate ofperipheral blood lymphocyte and the rates of MN , NPB and NB respectively (rS were 0.730, 0.515 and 0.660, P <0.01), but no statistical correlation was found between the b /c rate and the OTM (rS =-0.120, P >0.05).Conclusion Low levels of BD occupational exposure may lead to the genetic damage in peripheral blood lymphocytes of workers ,which may have some relevance with the DRC.