CAJ | 학술논문

目的：对比分析食管鳞癌组织和癌旁组织，食管鳞癌患者与正常人的血浆，检测COL14A1的甲基化频率，并结合患者的临床病理特征，分析COL14A1基因甲基化与食管鳞癌的临床特征的关系。方法收集42例食管鳞癌组织标本和相对应的癌旁组织标本，同时收集42例食管鳞癌患者和50例正常人的血浆标本；应用甲基化特异性PCR（MSP）结合琼脂糖凝胶电泳检测COL14A1在组织和血浆中的甲基化情况。结果 COL14A1在肿瘤组织中的甲基化频率为45.24%，显著高于癌旁组织11.9%（P<0.001）。COL14A1在肿瘤患者血浆中的甲基化频率为30.95%，在正常对照组的血浆中未检测到甲基化。组织中的COL14A1甲基化频率在病理分期为N2组明显高于病理分期N0-N1组。血浆中的COL14A1甲基化频率在病理分期T3组明显高于病理分期T1~T2组。结论 COL14A1在食管鳞癌组织的甲基化频率高于癌旁组织，在食管鳞癌患者血浆中的甲基化频率显著高于正常人，且临床分期越晚的甲基化频率越高。
목적：대비분석식관린암조직화암방조직，식관린암환자여정상인적혈장，검측COL14A1적갑기화빈솔，병결합환자적림상병리특정，분석COL14A1기인갑기화여식관린암적림상특정적관계。방법수집42례식관린암조직표본화상대응적암방조직표본，동시수집42례식관린암환자화50례정상인적혈장표본；응용갑기화특이성PCR（MSP）결합경지당응효전영검측COL14A1재조직화혈장중적갑기화정황。결과 COL14A1재종류조직중적갑기화빈솔위45.24%，현저고우암방조직11.9%（P<0.001）。COL14A1재종류환자혈장중적갑기화빈솔위30.95%，재정상대조조적혈장중미검측도갑기화。조직중적COL14A1갑기화빈솔재병리분기위N2조명현고우병리분기N0-N1조。혈장중적COL14A1갑기화빈솔재병리분기T3조명현고우병리분기T1~T2조。결론 COL14A1재식관린암조직적갑기화빈솔고우암방조직，재식관린암환자혈장중적갑기화빈솔현저고우정상인，차림상분기월만적갑기화빈솔월고。
Objective To detect COL14A1 gene methylation frequency difference between esophageal squamous cell carcinoma(ESCC) tissues and control normal tissues,between ESCC patients’plasma and healthy volenteers’ plasma,and to analyze the correlation with clinico-pathological parameters. Methods We collected esophageal squamous cell carcinoma tissues(n=42 cases)and adjacent surrounding normal tissues (n=42 cases),plasma of the esophageal squamous cell carcinoma(n=42 cases)and plasma of the healthy individuals(n=50 cases). We used Methylation specific PCR(MSP)combined with agarose gel electrophoresis to detect the methylation status of the COL14A1. We used SPSS 13.0 software for statistical analysis byχ2 test and Fisher’s exact test. Results GPX3 frequency of methylation in tumor tissue was 54.8%,significantly higher than the adjacent tissues which was 9.5%,the difference was statistically significant(χ2=28.873,P<0.001,n=84). In the plasma of cancer patients GPX3 methylation frequency was 40.5%,while the methylation of COL14A1 in the plasma of healthy volunteers was not detected. Methylation of GPX3 in tissue was more frequently seen in patients with T3 or N2 than in patients with T1-2 or N0-1. Conclusion The methylation frequency of COL14A1 is higher in ESCC tissus than in control normal tissues,and higer in plasma of ESCC patients than in healthy volunteers. GPX3 methylation was more frequently seen in patients with more advanced disease.