CAJ | 학술논문

目的：观察丙泊酚麻醉对新生小鼠海马星形胶质细胞（AST）和小胶质细胞的影响。方法将健康同窝日龄7d（P7）C57小鼠15只，随机分为丙泊酚高剂量组、低剂量组和10％脂肪乳对照组，每组5只，所有小鼠从P7开始接受药物处理。高、低剂量组分别腹腔注射丙泊酚60、30mg／kg；对照组腹腔注射同等体积的10％脂肪乳。药物处理24h后取材，采用免疫组化方法检测AST标记物胶质纤维酸性蛋白（GFAP）与小胶质细胞标记物离子钙接头分子（Iba1）在海马内的表达。结果丙泊酚高剂量组小鼠海马齿状回分子层中GFAP标记的AST数量较对照组明显减少（P＜0．01），低剂量丙泊酚对新生鼠海马中AST数量无显著影响；高剂量和低剂量丙泊酚均显著性降低海马小胶质细胞数量（P＜0．01）。结论丙泊酚抑制新生小鼠海马AST和小胶质细胞的发育，且存在剂量依赖关系。
목적：관찰병박분마취대신생소서해마성형효질세포（AST）화소효질세포적영향。방법장건강동와일령7d（P7）C57소서15지，수궤분위병박분고제량조、저제량조화10％지방유대조조，매조5지，소유소서종P7개시접수약물처리。고、저제량조분별복강주사병박분60、30mg／kg；대조조복강주사동등체적적10％지방유。약물처리24h후취재，채용면역조화방법검측AST표기물효질섬유산성단백（GFAP）여소효질세포표기물리자개접두분자（Iba1）재해마내적표체。결과병박분고제량조소서해마치상회분자층중GFAP표기적AST수량교대조조명현감소（P＜0．01），저제량병박분대신생서해마중AST수량무현저영향；고제량화저제량병박분균현저성강저해마소효질세포수량（P＜0．01）。결론병박분억제신생소서해마AST화소효질세포적발육，차존재제량의뢰관계。
Objective To observe the effects of propofol on the hippocampal astrocytes and microglia in the nenotal mice . Methods 15 healthy mice from the same litters on postnatal 7 d were randomized into 3 groups:high dose propofol group ,low dose propofol group and 10% intralipid control group .All mice were treated with drugs on postnatal 7 d by intraperitoneal injection and were sacrificed at 24 h after drugs treatment .The high dose group was injected with propofol 60mg · kg -1 ;the low dose group was injected with propofol 30mg · kg -1 ;the control group was injected with the equal volume of 10% intralipid .The immunohistochem‐istry assay was used to detect the expression of glial fibrillary acidic protein (GFAP) and ionized calcium binding adapter molecular 1 (Iba1) for observing the effect of propofol on the astrocytes (AST ) and microglia in the hippocampus .Results Compared with the control group ,the number of GFAP‐labeled AST in the dentate gyms (DG) molecular layer of hippocampus in P7 mice of the high dose propofol group was significantly reduced (P<0 .01) ,while no obvious effect of the low‐dose propofol on the number of AST was observed ;high dose and low dose propofol all significantly decreased the number of Iba1‐labeled microglia .Conclusion Propofol can inhibit the growth of the hippocampal AST and microglia in a dose‐dependent manner .